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Scanning near-field optical microscopy and microspectroscopy of green fluorescent protein in intact Escherichia coli bacteria

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Subramaniam,  V.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Kirsch,  A. K.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Rivera-Pomar,  R.
Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Jovin,  T. M.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Subramaniam, V., Kirsch, A. K., Rivera-Pomar, R., & Jovin, T. M. (1997). Scanning near-field optical microscopy and microspectroscopy of green fluorescent protein in intact Escherichia coli bacteria. Journal of Fluorescence, 7(4), 381-385.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-0068-1
Abstract
Scanning near-field optical microscopy (SNOM) yields high-resolution topographic and optical information and constitutes an important new technique for visualizing biological systems. By coupling a spectrograph to a near-field microscope, we have been able to perdorem microspectroscopic measurements with a spatial resolution greatly exceeding that of the conventional optical microscope. Here we present SNO images of Escherischia coli bacteria expressing a mutant green fluorescent protein (GFP), an important reporter molecule in the cell, developmental, and molecular biology. Near-field emission spectra confirm that the fluorescence detected by SNOM arises from bacterially expressed GFP molecules.