Swelling-induced catecholamine secretion recorded from single chromaffin cells

Moser, T.; Chow, R. H.; Neher, E.

doi:10.1007/BF00410191

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Swelling-induced catecholamine secretion recorded from single chromaffin cells

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Moser, T.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Chow, R. H.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Neher, E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Moser, T., Chow, R. H., & Neher, E. (1995). Swelling-induced catecholamine secretion recorded from single chromaffin cells. Pflügers Archiv: European Journal of Physiology, 431(2), 196-203. doi:10.1007/BF00410191.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0013-024E-F

Abstract

We have studied osmotically induced catecholamine secretion from bovine adrenal chromaffin cells by combining patch-clamp measurements, electrochemical detection of secretion, and Fura-2 measurements of intracellular free calcium concentration ([Ca2+]i). We find that osmotically induced catecholamine release is exocytotic and calcium dependent. Furthermore, we demonstrate that cell swelling is coupled to such secretion via a volume-activated current, carrying predominantly chloride, which causes a plateau depolarization of the cell membrane potential and thus promotes voltage-activated calcium influx. Therefore, cell volume changes may modulate the secretory activity.