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Journal Article

Lack of direct evidence for a functional role of voltage-operated calcium channels in juxtaglomerular cells.

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Penner,  R.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Kurtz, A., Skott, O., Chegini, S., & Penner, R. (1990). Lack of direct evidence for a functional role of voltage-operated calcium channels in juxtaglomerular cells. Pflügers Archiv-European Journal of Physiology, 416(3), 281-287. doi:10.1007/BF00392064.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-0DE1-D
Abstract
In this study we have examined the role of voltage-gated calcium channels in the regulation of calcium in juxtaglomerular cells. Using a combination of patch-clamp and single-cell calcium measurement we obtained evidence neither for voltage-operated calcium currents nor for changes of the intracellular calcium concentration upon acute depolarizations of the cell membrane. Increases of the extracellular concentration of potassium to 80 mmol/l depolarized the juxtaglomerular cells close to the potassium equilibrium potential, but did not alter the intracellular calcium concentration neither in patch-clamped nor in intact Furaester-loaded cells. Moreover, basal renin secretion from a preparation enriched in mouse juxtaglomerular cells and from rat glomeruli with attached juxtaglomerular cells was not inhibited when extracellular potassium was isoosmotically increased to 56 mmol/l. In mouse kidney slices, however, depolarizing potassium concentrations caused a delayed inhibition at 56 mmol/l and a delayed stimulation of renin secretion at 110 mmol/l. Taken together, our study does not provide direct evidence for a role of voltage-activated calcium channels in the regulation of calcium and renin secretion in renal juxtaglomerular cells.