Novel red fluorophores with superior performance in STED microscopy.

Wurm, C. A.; Kolmakov, K.; Göttfert, F.; Ta, H.; Bossi, M. L.; Schill, H.; Berning, S.; Jakobs, S.; Donnert, G.; Belov, V. N.; Hell, S. W.

doi:10.1186/2192-2853-1-7

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Journal Article

Novel red fluorophores with superior performance in STED microscopy.

MPS-Authors

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Wurm, C. A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Kolmakov, K.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Bossi, M. L.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Schill, H.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Berning, S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15269

Jakobs, S.
Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15002

Donnert, G.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Belov, V. N.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell, S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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1563873-Suppl.doc
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Citation

Wurm, C. A., Kolmakov, K., Göttfert, F., Ta, H., Bossi, M. L., Schill, H., et al. (2012). Novel red fluorophores with superior performance in STED microscopy. Optical Nanoscopy, 1: 7. doi:10.1186/2192-2853-1-7.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-192A-D

Abstract

In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells.