Artist: mastermindxberg date: 2010-07-01T11:51:10 Fill Order: Normal Compression: LZW By-line: mastermindxberg Samples Per Pixel: 3 samples/pixel Inter Color Profile: [3144 values] Strip Offsets: [1410 values] tiff:ResolutionUnit: Inch Object Name: Plos Figures Cav1.cdr X Resolution: 300 dots per inch Photometric Interpretation: RGB dc:title: Figure S1 modified: 2010-07-01T11:51:10 tiff:BitsPerSample: 8 Application Record Version: 0 meta:author: mastermindxberg meta:creation-date: 2010-07-01T11:51:10 tiff:SamplesPerPixel: 3 exif:PageCount: 1 Creation-Date: 2010-07-01T11:51:10 Orientation: Top, left side (Horizontal / normal) tiff:Orientation: 1 tiff:Software: Adobe Photoshop CS3 Windows XMP Value Count: 15 Author: mastermindxberg Software: Adobe Photoshop CS3 Windows tiff:YResolution: 300.0 Y Resolution: 300 dots per inch dc:description:

The inhibitory effect of Cav1 on P+GC internalization is pili-specific and independent of pili retraction. (A) Attachment of non-piliated P?Opa57+GC does not induce Cav1 (white, middle panel) recruitment 2 h post-infection. (B) Expression of Cav1 in AGS cells does not inhibit P?Opa57+GC internalization. Mock-transfected AGS cells and AGS-Cav1 cells were infected with P+GC or P?Opa57+GC. (C) Cav1 inhibits uptake of the P+GC?pilT mutant. AGS-Mock and AGS-Cav1 cells were infected with P+GC or the P+GC?pilT mutant. Gentamicin protection assays were performed 2 h post-infection. Intracellular gentamicin protected (GmP) bacteria were determined as a percentage of total cell-associated bacteria, which were comparable for the different host cells. Experiments were performed in triplicate. Error bars indicate mean ± standard deviation.

(0.36 MB TIF)

Predictor: 2 dc:creator: mastermindxberg tiff:ImageLength: 1410 description:

The inhibitory effect of Cav1 on P+GC internalization is pili-specific and independent of pili retraction. (A) Attachment of non-piliated P?Opa57+GC does not induce Cav1 (white, middle panel) recruitment 2 h post-infection. (B) Expression of Cav1 in AGS cells does not inhibit P?Opa57+GC internalization. Mock-transfected AGS cells and AGS-Cav1 cells were infected with P+GC or P?Opa57+GC. (C) Cav1 inhibits uptake of the P+GC?pilT mutant. AGS-Mock and AGS-Cav1 cells were infected with P+GC or the P+GC?pilT mutant. Gentamicin protection assays were performed 2 h post-infection. Intracellular gentamicin protected (GmP) bacteria were determined as a percentage of total cell-associated bacteria, which were comparable for the different host cells. Experiments were performed in triplicate. Error bars indicate mean ± standard deviation.

(0.36 MB TIF)

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