Artist: mastermindxberg date: 2010-07-01T11:51:30 Fill Order: Normal Compression: LZW By-line: mastermindxberg Samples Per Pixel: 3 samples/pixel Inter Color Profile: [3144 values] Strip Offsets: [1453 values] tiff:ResolutionUnit: Inch Object Name: Plos Figures Cav1.cdr X Resolution: 300 dots per inch Photometric Interpretation: RGB dc:title: Figure S2 modified: 2010-07-01T11:51:30 tiff:BitsPerSample: 8 Application Record Version: 0 meta:author: mastermindxberg meta:creation-date: 2010-07-01T11:51:30 tiff:SamplesPerPixel: 3 exif:PageCount: 1 Creation-Date: 2010-07-01T11:51:30 Orientation: Top, left side (Horizontal / normal) tiff:Orientation: 1 tiff:Software: Adobe Photoshop CS3 Windows XMP Value Count: 15 Author: mastermindxberg Software: Adobe Photoshop CS3 Windows tiff:YResolution: 300.0 Y Resolution: 300 dots per inch dc:description:

Tfp-producing EPEC induce Cav1 accumulation and prevent host cell entry. (A) ME-180 cells were infected with pre-activated cultures of wild-type EPEC strain E2348/69 and EPEC 2348/69 CVD452, a type III secretion system (TTSS) defective mutant, for 2 h. Endogenous Cav1 (green) is recruited to attachment sites of the microcolony-forming mutant, whereas the TTSS-preactivated wild type adheres dispersed and does not trigger Cav1 recruitment. (B) AGS and AGS-Cav1 cells were infected with E2248/69 wild type and TTSS CVD452 mutant EPEC. Intracellular gentamicin protected (GmP) bacteria were determined as a percentage of total cell-associated bacteria. Experiments were performed in triplicate. Error bars indicate mean ± standard deviation.

(0.89 MB TIF)

Predictor: 2 dc:creator: mastermindxberg tiff:ImageLength: 1453 description:

Tfp-producing EPEC induce Cav1 accumulation and prevent host cell entry. (A) ME-180 cells were infected with pre-activated cultures of wild-type EPEC strain E2348/69 and EPEC 2348/69 CVD452, a type III secretion system (TTSS) defective mutant, for 2 h. Endogenous Cav1 (green) is recruited to attachment sites of the microcolony-forming mutant, whereas the TTSS-preactivated wild type adheres dispersed and does not trigger Cav1 recruitment. (B) AGS and AGS-Cav1 cells were infected with E2248/69 wild type and TTSS CVD452 mutant EPEC. Intracellular gentamicin protected (GmP) bacteria were determined as a percentage of total cell-associated bacteria. Experiments were performed in triplicate. Error bars indicate mean ± standard deviation.

(0.89 MB TIF)

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