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dc:title: Figure S1
modified: 2009-09-07T20:57:17
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dc:description: <p>Knockdown of Rab proteins. A) Analysis of KD efficiency after siRNA treatment via quantitative real time PCR at 48 h <italic>p.t.</italic> Diagram shows KD efficiency in percent to control cells. B) HeLa cells were transfected with siRNAs against Rab6A, Rab11A and Luciferase (control). At 3 d <italic>p.t.</italic>, cells were lysed in RIPA buffer and proteins were separated by SDS-PAGE. Proteins were transferred to PVDF membranes followed by immunoblot with antibodies specific for Rab6 and Rab11 and actin. Actin was used as loading control. Blots are representative of n?=?3. C) Three days <italic>p.t.</italic> Rab6A and Rab11A siRNA-treated Hep-2 cells were infected with <italic>C. trachomatis</italic> (MOI 3). At 44 h <italic>p.i.</italic>, cells were lysed and infectious bacteria were titrated on HeLa cells. Numbers of infectious bacteria in KD cells were expressed as IFU (inclusion forming units). shRNA against Luciferase was used as a control. (A, C) Values were obtained from three independent experiments and are meanąSE. Student's t-test was performed to determine p-value, *<0.05.</p>
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description: <p>Knockdown of Rab proteins. A) Analysis of KD efficiency after siRNA treatment via quantitative real time PCR at 48 h <italic>p.t.</italic> Diagram shows KD efficiency in percent to control cells. B) HeLa cells were transfected with siRNAs against Rab6A, Rab11A and Luciferase (control). At 3 d <italic>p.t.</italic>, cells were lysed in RIPA buffer and proteins were separated by SDS-PAGE. Proteins were transferred to PVDF membranes followed by immunoblot with antibodies specific for Rab6 and Rab11 and actin. Actin was used as loading control. Blots are representative of n?=?3. C) Three days <italic>p.t.</italic> Rab6A and Rab11A siRNA-treated Hep-2 cells were infected with <italic>C. trachomatis</italic> (MOI 3). At 44 h <italic>p.i.</italic>, cells were lysed and infectious bacteria were titrated on HeLa cells. Numbers of infectious bacteria in KD cells were expressed as IFU (inclusion forming units). shRNA against Luciferase was used as a control. (A, C) Values were obtained from three independent experiments and are meanąSE. Student's t-test was performed to determine p-value, *<0.05.</p>
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dcterms:created: 2009-09-07T20:57:17
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title: Figure S1
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