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High-density microcarrier cell cultures for influenza virus production

MPG-Autoren
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Bock,  A.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Schulze-Horsel,  J.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Schwarzer,  J.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Rapp,  E.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Genzel,  Y.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Reichl,  U.
Otto-von-Guericke-Universität Magdeburg;
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Zitation

Bock, A., Schulze-Horsel, J., Schwarzer, J., Rapp, E., Genzel, Y., & Reichl, U. (2011). High-density microcarrier cell cultures for influenza virus production. Biotechnology Progress, 27(1), 241-250. doi:10.1002/btpr.539.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0013-8CD1-F
Zusammenfassung
Influenza virus A/PR/8/34 virus propagation in adherent Madin-Darby canine kidney cells in high-density microcarrier cultures is described. To improve virus yields perfusion and repeated fed-batch modes were applied using cell-specific feed rates. Cell densities up to 1.1 x 107 cells/mL were achieved. Cell-specific virus yields in high-density cultures were at similar level compared to standard, low-density cultivations. In the average 2400 and 3300 virions per cell were obtained for two variants of the virus strain A/PR/8/34, PR8-NIBSC and PR8-RKI, respectively. Maximum virus titer (HA-activity = 1778 HAU/100μL) for virus variant PR8-NIBSC was obtained for a cultivation infected before maximum cell concentration was reached.

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