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dc:title: Figure S5
modified: 2013-06-25T17:50:28
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dc:description: Loss of Geminin does not cause cell cycle aberrations or apoptosis in MEFs. A) Gmnn fl/fl; ER-Cre and Gmnn fl/+; ER-Cre MEFs were treated with tamoxifen for 48 hours. Whole cell lysate was run on the SDS-PAGE gels and geminin was immunobloted. The amount of loaded protein was controlled by Tubulin. B) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours, and analyzed with flow cytometry. C) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours and immuno-stained for phosho-histone 3, the M phase marker. In addition to tamoxifen MEFs received a 4 hours pulse of BrdU to label the cells in the S phase and were stained for BrdU in order to visualize the S phase. D) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours and immuno-stained for cyclins. Cells were counted and abundances were calculated relative to total number of the cells. E) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours and immuno-stained for Ki67,a marker for proliferating cells. Cells were counted and abundances were calculated relative to total number of the cells. F) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours and stained for TUNEL (apoptosis marker). Treated cells were counted and the percentage of positive cells is represented in the graph.(TIF)
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description: Loss of Geminin does not cause cell cycle aberrations or apoptosis in MEFs. A) Gmnn fl/fl; ER-Cre and Gmnn fl/+; ER-Cre MEFs were treated with tamoxifen for 48 hours. Whole cell lysate was run on the SDS-PAGE gels and geminin was immunobloted. The amount of loaded protein was controlled by Tubulin. B) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours, and analyzed with flow cytometry. C) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours and immuno-stained for phosho-histone 3, the M phase marker. In addition to tamoxifen MEFs received a 4 hours pulse of BrdU to label the cells in the S phase and were stained for BrdU in order to visualize the S phase. D) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours and immuno-stained for cyclins. Cells were counted and abundances were calculated relative to total number of the cells. E) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours and immuno-stained for Ki67,a marker for proliferating cells. Cells were counted and abundances were calculated relative to total number of the cells. F) fl/+ and fl/fl MEFs were treated with tamoxifen for 48 hours and stained for TUNEL (apoptosis marker). Treated cells were counted and the percentage of positive cells is represented in the graph.(TIF)
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dcterms:created: 2013-06-25T17:50:28
dcterms:modified: 2013-06-25T17:50:28
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title: Figure S5
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