Fill Order: Normal Compression: LZW dc:description: Time course of labelling after electroporation and influence of electroporation on the growth rate. (A) Living yeast cells expressing mtHalo were labelled using the commercially available TMR-Halo substrate by electroporation. Subsequently the cells were cultivated at 30 °C and imaged every 30 minutes by epifluorescence microscopy. (B) Growth curve of cells after electroporation. Cells were subjected to electroporation with or without TMR-Halo, or not challenged. Electroporation settings: 1000 V, 800 ?, 25 µF. Scale bar: 2 µm.(TIF) Predictor: 2 tiff:ImageLength: 737 description: Time course of labelling after electroporation and influence of electroporation on the growth rate. (A) Living yeast cells expressing mtHalo were labelled using the commercially available TMR-Halo substrate by electroporation. Subsequently the cells were cultivated at 30 °C and imaged every 30 minutes by epifluorescence microscopy. (B) Growth curve of cells after electroporation. Cells were subjected to electroporation with or without TMR-Halo, or not challenged. Electroporation settings: 1000 V, 800 ?, 25 µF. Scale bar: 2 µm.(TIF) Page Number: 0 1 Samples Per Pixel: 3 samples/pixel Strip Offsets: [737 values] tiff:ResolutionUnit: Inch title: Figure S3 X Resolution: 300 dots per inch Planar Configuration: Chunky (contiguous for each subsampling pixel) Photometric Interpretation: RGB File Size: 728659 bytes Rows Per Strip: 1 rows/strip dc:title: Figure S3 File Name: apache-tika-7581075989530806556.tmp tiff:BitsPerSample: 8 Content-Type: image/tiff Strip Byte Counts: 152 152 152 152 152 152 152 152 152 152 152 152 152 152 436 607 607 559 577 577 592 592 565 620 620 555 626 619 652 649 652 690 685 631 694 726 717 730 685 731 714 727 926 956 942 1057 1026 1021 1018 1032 1057 1051 960 962 967 992 1008 1006 1068 1019 1101 1145 1101 1129 1158 982 919 917 934 945 952 951 938 892 927 895 969 997 963 1011 1014 975 1026 1026 1117 1121 1098 1184 1167 1135 1213 1186 1190 1217 1156 1157 1165 1167 1252 1268 1255 1241 1179 1219 1187 1197 1238 1127 1102 1101 1084 1132 1118 1110 1169 1161 1153 1146 1106 1165 1175 1138 1178 1143 1160 1187 1213 1285 1314 1275 1271 1272 1294 1286 1237 1256 1249 1224 1252 1238 1278 1312 1264 1316 1327 1271 1278 1283 1266 1245 1191 1216 1222 1197 1227 1205 1248 1202 1202 1220 1237 1253 1235 1239 1245 1249 1213 1227 1233 1234 1245 1226 1266 1272 1239 1296 1259 1250 1252 1255 1266 1248 1198 1256 1274 1217 1261 1239 1271 1268 1246 1241 1255 1250 1253 1230 1231 1223 1195 1226 1206 1180 1230 1198 1245 1248 1205 1244 1250 1222 1233 1227 1234 1205 1176 1206 1205 1160 1194 1169 1217 1194 1142 1172 1176 1176 1157 1153 1150 1151 1084 1165 1160 1105 1142 1062 1094 1073 1017 1055 1073 1092 1090 1070 1098 1077 1022 1125 1138 1030 1099 1035 1063 1061 1037 1103 1085 1021 1025 1007 1007 1015 964 1007 981 980 1002 951 986 978 907 936 925 933 934 944 937 936 900 905 901 877 887 862 839 821 794 836 845 905 911 916 827 832 819 846 836 802 852 787 802 786 771 936 917 905 953 1061 1052 1035 955 1026 1102 1008 947 877 1010 984 871 884 890 906 914 912 884 899 844 886 881 879 909 889 861 889 876 894 782 791 845 749 639 642 630 631 581 545 570 546 162 162 165 165 170 161 161 188 178 176 184 195 202 195 196 194 196 203 203 202 194 192 187 670 670 702 707 706 752 736 719 715 714 710 786 786 814 824 820 944 944 925 864 872 955 984 964 1011 980 967 1011 1002 1084 1084 1077 1063 1037 1047 1092 1094 1081 1124 1106 1138 1134 1139 1198 1201 1135 1099 1091 1105 1103 1117 1190 1187 1147 1143 1147 1154 1171 1151 1145 1145 1145 1149 1149 1173 1191 1165 1171 1168 1160 1180 1156 1216 1230 1205 1233 1227 1252 1274 1270 1228 1250 1209 1227 1230 1208 1263 1238 1220 1228 1212 1259 1274 1266 1268 1282 1270 1253 1261 1279 1242 1219 1239 1217 1227 1227 1234 1238 1228 1213 1213 1213 1213 1222 1186 1186 1191 1197 1217 1208 1212 1206 1189 1197 1211 1191 1180 1189 1172 1184 1162 1171 1175 1160 1179 1161 1180 1175 1183 1209 1219 1172 1169 1158 1180 1176 1179 1189 1194 1197 1204 1184 1167 1171 1161 1180 1175 1153 1134 1128 1165 1179 1143 1140 1167 1134 1154 1140 1109 1125 1112 1118 1120 1114 1103 1106 1154 1189 1139 1127 1107 1092 1101 1101 1118 1117 1106 1128 1125 1107 1107 1117 1096 1085 1091 1105 1091 1065 1099 1083 1072 1062 1059 1076 1063 1083 1084 1080 1041 1019 1011 1028 1066 1058 1054 1068 1051 1055 1051 1076 1058 1062 1055 1047 1065 1018 1015 1033 1047 1006 1080 1080 1110 1103 1091 1129 1080 1044 1055 1050 1043 1048 992 991 997 959 978 980 989 995 980 999 985 1041 1041 1044 1057 1057 960 973 1238 1169 1171 1256 1266 1263 1231 1219 1233 1242 1220 1121 1139 1132 1178 1176 1180 1138 1118 1151 1189 1183 1180 1197 1161 1048 1239 1142 1002 1014 1190 1154 1158 1072 1101 1150 1134 1062 1092 1040 1044 905 966 885 930 955 895 881 917 845 877 855 899 1014 934 969 978 1112 1094 889 931 948 871 744 750 784 789 764 725 737 740 737 740 312 268 263 261 259 173 173 173 173 182 170 170 170 170 152 152 152 152 152 152 152 152 152 152 152 152 152 152 152 152 152 152 152 bytes X-Parsed-By: org.apache.tika.parser.DefaultParser File Modified Date: Thu Apr 25 17:57:10 +02:00 2024 tiff:XResolution: 300.0 tiff:SamplesPerPixel: 3 Document Name: /tmp/tmp_4849674000160537620pone.0078745.s003.tif exif:PageCount: 1 Image Height: 737 pixels Orientation: Top, left side (Horizontal / normal) tiff:Orientation: 1 Image Width: 2847 pixels Resolution Unit: Inch XMP Value Count: 15 tiff:ImageWidth: 2847 tiff:YResolution: 300.0 Bits Per Sample: 8 8 8 bits/component/pixel Y Resolution: 300 dots per inch