date: 2013-10-18T14:00:45Z
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pdf:docinfo:title: Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement
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dc:description: The RNA exosome is an evolutionarily conserved multi-protein complex involved in the 3' degradation of a variety of RNA transcripts. In the nucleus, the exosome participates in the maturation of structured RNAs, in the surveillance of pre-mRNAs and in the decay of a variety of noncoding transcripts. In the cytoplasm, the exosome degrades mRNAs in constitutive and regulated turnover pathways. Several structures of subcomplexes of eukaryotic exosomes or related prokaryotic exosome-like complexes are known, but how the complete assembly is organized to fulfil processive RNA degradation has been unclear. An atomic snapshot of a Saccharomyces cerevisiae 420 kDa exosome complex bound to an RNA substrate in the pre-cleavage state of a hydrolytic reaction has been determined. Here, the crystallographic steps towards the structural elucidation, which was carried out by molecular replacement, are presented.
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description: The RNA exosome is an evolutionarily conserved multi-protein complex involved in the 3' degradation of a variety of RNA transcripts. In the nucleus, the exosome participates in the maturation of structured RNAs, in the surveillance of pre-mRNAs and in the decay of a variety of noncoding transcripts. In the cytoplasm, the exosome degrades mRNAs in constitutive and regulated turnover pathways. Several structures of subcomplexes of eukaryotic exosomes or related prokaryotic exosome-like complexes are known, but how the complete assembly is organized to fulfil processive RNA degradation has been unclear. An atomic snapshot of a Saccharomyces cerevisiae 420 kDa exosome complex bound to an RNA substrate in the pre-cleavage state of a hydrolytic reaction has been determined. Here, the crystallographic steps towards the structural elucidation, which was carried out by molecular replacement, are presented.
dcterms:created: 2013-10-12T12:00:00Z
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dcterms:modified: 2013-10-18T14:00:45Z
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title: Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement
Last-Save-Date: 2013-10-18T14:00:45Z
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dc:title: Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement
modified: 2013-10-18T14:00:45Z
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Creation-Date: 2013-10-12T12:00:00Z
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producer: International Union of Crystallography
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pdf:docinfo:producer: International Union of Crystallography
pdf:docinfo:created: 2013-10-12T12:00:00Z