Two-color RESOLFT nanoscopy with green and red fluorescent photochromic 
proteins.

Lavoie-Cardinal, F.; Jensen, N. A.; Westphal, V.; Stiel, A. C.; Chmyrov, A.; Bierwagen, J.; Testa, I.; Jakobs, S.; Hell, S. W.

doi:10.1002/cphc.201301016

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学術論文

Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins.

MPS-Authors

/persons/resource/persons39415

Lavoie-Cardinal, F.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons128160

Jensen, N. A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons16012

Westphal, V.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15874

Stiel, A. C.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons98970

Chmyrov, A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons14854

Bierwagen, J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons39411

Testa, I.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15269

Jakobs, S.
Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15210

Hell, S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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1938090.pdf
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1938090_Suppl.pdf
(付録資料), 4MB

引用

Lavoie-Cardinal, F., Jensen, N. A., Westphal, V., Stiel, A. C., Chmyrov, A., Bierwagen, J., Testa, I., Jakobs, S., & Hell, S. W. (2014). Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins. ChemPhysChem, 15(4), 655-663. doi:10.1002/cphc.201301016.

引用: https://hdl.handle.net/11858/00-001M-0000-0015-82CA-2

要旨

Up to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly switchable fluorescent proteins (RSFP) emitting in the green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red-emitting RSFP enabling a spatial resolution up to four times higher than the diffraction barrier. By co-expressing green and red RSFPs in living cells we demonstrate two-color RESOLFT imaging both for single (“donut”) beam scanning and for parallelized versions of RESOLFT nanoscopy where an array of >23 000 “donut-like” minima are scanned simultaneously.