Extended Data Table 2: Resistant mutations in proteins of the yeast ribosome
From Structural basis for the inhibition of the eukaryotic ribosome
- Nicolas Garreau de Loubresse1,
- Irina Prokhorova1,
- Wolf Holtkamp2,
- Marina V. Rodnina2,
- Gulnara Yusupova1,
- Marat Yusupov1,
- Journal name:
- Nature
- Volume:
- 513,
- Pages:
- 517–522
- Date published:
- DOI:
- doi:10.1038/nature13737
Additional data
Affiliations
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Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964, CNRS UMR7104, Université de Strasbourg, 67404, Illkirch, France
- Nicolas Garreau de Loubresse,
- Irina Prokhorova,
- Gulnara Yusupova &
- Marat Yusupov
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Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
- Wolf Holtkamp &
- Marina V. Rodnina
Contributions
M.Y. supervised the study. N.G.D.L. designed the experiments. N.G.D.L. and I.P. conducted purification, crystallization and post-crystallization treatment experiments, collected X-ray diffraction data and carried out the structure determination. N.G.D.L., I.P., G.Y. and M.Y. analysed the crystal structures. M.V.R. and W.H. designed, performed and interpreted rapid kinetic experiments. N.G.D.L. wrote the initial manuscript to which M.V.R., G.Y. and M.Y. contributed specialist insights. All authors helped with refining the manuscript and approved the final version.
Competing financial interests
The authors declare no competing financial interests.
Atomic coordinates and structure factors for the reported crystal structures have been deposited in the Protein Data Bank (http://www.pdb.org/pdb/home/home.do) under accession codes 4U3M (anisomycin), 4U56 (blasticidin S), 4U3N (CCA), 4U55 (cryptopleurine), 4U3U (cycloheximide), 4U53 (deoxynivalenol), 4U4N (edeine), 4U4O (geneticin G418), 4U4Q (homoharringtonine), 4U4R (lactimidomycin), 4U4U (lycorine), 4U52 (nagilactone C), 4U51 (narcilasine), 4U4Y (pactamycin), 4U4Z (phyllanthoside), 4U6F (T-2 toxin) and 4U50 (verrucarin).
Author details
Nicolas Garreau de Loubresse
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Irina Prokhorova
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Wolf Holtkamp
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Marina V. Rodnina
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Gulnara Yusupova
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Marat Yusupov
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Extended Data Figure 1: Unbiased positive electron density of small-molecule inhibitors and CCA-trinucleotide.Hover over figure to zoom
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Extended Data Figure 2: Electron density of small-molecule inhibitors and CCA-trinucleotide.Hover over figure to zoom
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Extended Data Figure 3: Structures of homoharringtonine, anisomycin, blasticidin S and pactamycin in eukaryotes, archaea and bacteria.Hover over figure to zoom
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Extended Data Figure 4: Structural differences in protein eL42 may preclude the binding of lactimidomycin and phyllanthoside to the archaeal ribosome.Hover over figure to zoom
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Extended Data Figure 5: Close-up view of CCA tri-nucleotide binding site.Hover over figure to zoom
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Extended Data Figure 6: Kinetic study of lactimidomycin and cycloheximide.Hover over figure to zoom
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Extended Data Figure 7: Close-up view of blasticidin S binding site.Hover over figure to zoom
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Extended Data Figure 8: Conformational changes in the peptidyl transferase centre and differences with the archaeal ribosome.Hover over figure to zoom
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Extended Data Table 1: Summary of information for the 16 small-molecule inhibitorsHover over figure to zoom
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Extended Data Table 2: Resistant mutations in proteins of the yeast ribosomeHover over figure to zoom