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dc:title: Figure S2
modified: 2014-08-28T09:29:13
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dc:description: In-vitro rates as measured for near-cognate accommodation and rejection after proofreading.In-vitro values of the rates  and  for near-cognate accommodation and rejection after proofreading at 20°C as determined by the experimental protocol described previously in Ref. [34]. The formation of f[3H]Met[14C]Phe was monitored under multiple-turnover conditions using initiation complexes 70S?mRNA(AUGCUC)?f[3H]Met-tRNAfMet (0.14 ?M) and varying concentrations of the ternary complex EF-Tu?GTP?[14C]Phe-tRNAPhe, which is near-cognate to the CUC codon. For each concentration of the ternary complex, the rates were determined from the linear slopes of the time courses. From the hyperbolic dependence of the concentration dependence of , we calculated s and  Using the previously measured efficiency  of the proofreading step [25], we then obtained the value s for near-cognate rejection after proofreading.(TIF)
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description: In-vitro rates as measured for near-cognate accommodation and rejection after proofreading.In-vitro values of the rates  and  for near-cognate accommodation and rejection after proofreading at 20°C as determined by the experimental protocol described previously in Ref. [34]. The formation of f[3H]Met[14C]Phe was monitored under multiple-turnover conditions using initiation complexes 70S?mRNA(AUGCUC)?f[3H]Met-tRNAfMet (0.14 ?M) and varying concentrations of the ternary complex EF-Tu?GTP?[14C]Phe-tRNAPhe, which is near-cognate to the CUC codon. For each concentration of the ternary complex, the rates were determined from the linear slopes of the time courses. From the hyperbolic dependence of the concentration dependence of , we calculated s and  Using the previously measured efficiency  of the proofreading step [25], we then obtained the value s for near-cognate rejection after proofreading.(TIF)
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dcterms:created: 2014-08-28T09:29:13
dcterms:modified: 2014-08-28T09:29:13
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title: Figure S2
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