Effects on capacitance by overexpression of membrane proteins

Zimmermann, D.; Zhou, A.; Kiesel, M.; Feldbauer, K.; Terpitz, U.; Haase, W.; Schneider-Hohendorf, T.; Bamberg, E.; Sukhorukov, V. L.

doi:10.1016/j.bbrc.2008.02.153

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Effects on capacitance by overexpression of membrane proteins

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Zimmermann, D.
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Feldbauer, K.
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Terpitz, U.
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Haase, W.
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Bamberg, E.
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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引用

Zimmermann, D., Zhou, A., Kiesel, M., Feldbauer, K., Terpitz, U., Haase, W., Schneider-Hohendorf, T., Bamberg, E., & Sukhorukov, V. L. (2008). Effects on capacitance by overexpression of membrane proteins. Biochemical and Biophysical Research Communications, 369(4), 1022-1026. doi:10.1016/j.bbrc.2008.02.153.

引用: https://hdl.handle.net/11858/00-001M-0000-0024-D85C-2

要旨

Functional Channelrhodopsin-2 (ChR2) overexpression of about 10(4)channels/mum(2) in the plasma membrane of HEK293 cells was studied by patch-clamp and freeze-fracture electron microscopy. Simultaneous electrorotation measurements revealed that ChR2 expression was accompanied by a marked increase of the area-specific membrane capacitance (C(m)). The C(m) increase apparently resulted partly from an enlargement of the size and/or number of microvilli. This is suggested by a relatively large C(m) of 1.15+/-0.08 microF/cm(2) in ChR2-expressing cells measured under isotonic conditions. This value was much higher than that of the control HEK293 cells (0.79+/-0.02 microF/cm(2)). However, even after complete loss of microvilli under strong hypoosmolar conditions (100 mOsm), the ChR2-expressing cells still exhibited a significantly larger C(m) (0.85+/-0.07 microF/cm(2)) as compared to non-expressing control cells (0.70+/-0.03 microF/cm(2)). Therefore, a second mechanism of capacitance increase may involve changes in the membrane permittivity and/or thickness due to the embedded ChR2 proteins.