Structural and functional self-organization of Photosystem II in grana 
thylakoids.

Kirchhoff, Helmut; Haase, Winfried; Haferkamp, Silvia; Schott, Thomas; Borinski, Mauricio; Kubitscheck, Ulrich; Rögner, Matthias

doi:10.1016/j.bbabio.2007.05.009

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Structural and functional self-organization of Photosystem II in grana thylakoids.

MPS-Authors

/persons/resource/persons137691

Haase, Winfried
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Kirchhoff, H., Haase, W., Haferkamp, S., Schott, T., Borinski, M., Kubitscheck, U., et al. (2007). Structural and functional self-organization of Photosystem II in grana thylakoids. Biochimica et Biophysica Acta, Bioenergetics, 1767(9), 1180-1188. doi:10.1016/j.bbabio.2007.05.009.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-D8BB-C

Abstract

The biogenesis of the well-ordered macromolecular protein arrangement of photosystem (PS)II and light harvesting complex (LHC)II in grana thylakoid membranes is poorly understood and elusive. In this study we examine the capability of self organization of this arrangement by comparing the PSII distribution and antenna organization in isolated untreated stacked thylakoids with restacked membranes after unstacking. The PS II distribution was deduced from freeze-fracture electron microscopy. Furthermore, changes in the antenna organization and in the oligomerization state of photosystem II were monitored by chlorophyll a fluorescence parameters and size analysis of exoplasmatic fracture face particles. Low-salt induced unstacking leads to a randomization and intermixing of the protein complexes. In contrast, macromolecular PSII arrangement as well as antenna organization in thylakoids after restacking by restoring the original solvent composition is virtually identical to stacked control membranes. This indicates that the supramolecular protein arrangement in grana thylakoids is a self-organized process.