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Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat

MPG-Autoren
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Sakmann,  Bert
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Wu,  Ling-Gang
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

Externe Ressourcen

http://www.sciencedirect.com/science/article/pii/S0896627300810296/pdfft?md5=7d5f28f8816e6d5d7403afb2530bae1a&pid=1-s2.0-S0896627300810296-main.pdf
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http://dx.doi.org/10.1016/S0896-6273(00)81029-6
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Zitation

Kay, A. R., Alfonso, A., Alford, S., Cline, H. T., Holgado, A. M., Sakmann, B., et al. (1999). Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat. Neuron, 24(4), 809-817. doi:10.1016/S0896-6273(00)81029-6.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0024-9FE2-3
Zusammenfassung
The fluorescent probe FM1-43 has been used extensively for imaging vesicle recycling; however, high nonspecific adsorption resulting in elevated background levels has precluded its use in certain tissues, notably brain slices. We have found that a sulfobutylated derivative of beta-cyclodextrin (ADVASEP-7) has a higher affinity for FM1-43 than the plasma membrane. ADVASEP-7 was used as a carrier to remove FM1-43 nonspecifically bound to the outer leaflet of the plasma membrane or extracellular molecules, significantly reducing background staining. This has enabled us to visualize synaptic vesicle recycling in the nematode C. elegans, intact lamprey spinal cord, and rat brain slices.