Extended Data Table 1: Data collection and model refinement
From Structure of the E. coli ribosome–EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM
- Niels Fischer1, n1
- Piotr Neumann2, n1
- Andrey L. Konevega3, 4, 5,
- Lars V. Bock6,
- Ralf Ficner2,
- Marina V. Rodnina5,
- Holger Stark1, 7,
- Journal name:
- Nature
- Year published:
- DOI:
- doi:10.1038/nature14275
Additional data
Author footnotes
These authors contributed equally to this work.
- Niels Fischer &
- Piotr Neumann
Affiliations
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3D Electron Cryomicroscopy Group, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
- Niels Fischer &
- Holger Stark
-
Abteilung Molekulare Strukturbiologie, Institut für Mikrobiologie und Genetik, GZMB, Georg-August Universität Göttingen, Justus-von Liebig Weg 11, 37077 Göttingen, Germany
- Piotr Neumann &
- Ralf Ficner
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Molecular and Radiation Biophysics Department, B.P. Konstantinov Petersburg Nuclear Physics Institute of National Research Centre ‘Kurchatov Institute’, 188300 Gatchina, Russia
- Andrey L. Konevega
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St Petersburg Polytechnic University, Polytechnicheskaya, 29, 195251 St Petersburg, Russia
- Andrey L. Konevega
-
Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
- Andrey L. Konevega &
- Marina V. Rodnina
-
Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
- Lars V. Bock
-
Department of 3D Electron Cryomicroscopy, Institute of Microbiology and Genetics, Georg-August Universität, 37077 Göttingen, Germany
- Holger Stark
Contributions
N.F. designed and performed cryo-EM experiments and data analysis. P.N. conceived and performed pseudo-crystallographic refinement and model validation and analysed data. A.L.K. prepared ribosome complexes. L.V.B. performed and analysed molecular dynamics simulations. All authors discussed the results. H.S. and N.F. conceived the project and wrote the paper with input from all authors.
Competing financial interests
The authors declare no competing financial interests.
The 2.9 Å cryo-EM map of the E. coli ribosome–EF-Tu complex has been deposited in the Electron Microscopy Data Bank with accession code EMD-2847, the coordinates of the atomic model have been deposited in the Protein Data Bank under accession code 5AFI.
Author details
Niels Fischer
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Piotr Neumann
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Andrey L. Konevega
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Lars V. Bock
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Ralf Ficner
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Marina V. Rodnina
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Holger Stark
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Extended Data Figure 1: Aberration-corrected cryo-EM.Hover over figure to zoom
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Extended Data Figure 2: Hierarchical sorting of ribosome particle images.Hover over figure to zoom
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Extended Data Figure 3: Resolution curves and model validation of the E. coli 70S ribosome–EF-Tu cryo-EM structure.Hover over figure to zoom
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Extended Data Figure 4: Modifications in rRNA. Comparison between cryo-EM and X-ray crystallography.Hover over figure to zoom
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Extended Data Figure 5: rRNA modifications in the E. coli 70S ribosome.Hover over figure to zoom
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Extended Data Figure 6: Visualization of structural dynamics of the ribosome by different approaches.Hover over figure to zoom
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Extended Data Figure 7: Structure of E. coli EF-Tu–Phe–tRNAPhe bound to the ribosome.Hover over figure to zoom
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Extended Data Figure 8: Cryo-EM densities for mobile proteins L9 and L31 and the arrangement of L9 in polysomes.Hover over figure to zoom
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Extended Data Table 1: Data collection and model refinementHover over figure to zoom
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Extended Data Table 2: Comparison of densities as observed in cryo-EM and X-ray crystallography by local cross-correlation*Hover over figure to zoom