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dc:title: S1 Fig
modified: 2014-11-17T19:58:09
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dc:description: Binding of Mincle to S. pneumoniae is serotype-specific.Binding of Mincle-Fc to different S. pneumoniae serotypes was analyzed by flow cytometry. Mincle-Fc and hFc (20 ?g/mL) were incubated with S. pneumoniae serotype 1, serotype 2 (D39), serotype 3 (PN36), and serotype 9N at a concentration of 3×108 cells/mL in lectin binding buffer. Bound fusion protein was detected by a PE-conjugated goat anti-hFc antibody. Results are shown as MFI values (mean + SEM) and are representative of three independent experiments (triplicates each). Significance is indicated by asterisks, *=p<0.05; **=p<0.01; ***=p<0.001; ****=p<0.0001; ns = non-significant.(TIF)
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description: Binding of Mincle to S. pneumoniae is serotype-specific.Binding of Mincle-Fc to different S. pneumoniae serotypes was analyzed by flow cytometry. Mincle-Fc and hFc (20 ?g/mL) were incubated with S. pneumoniae serotype 1, serotype 2 (D39), serotype 3 (PN36), and serotype 9N at a concentration of 3×108 cells/mL in lectin binding buffer. Bound fusion protein was detected by a PE-conjugated goat anti-hFc antibody. Results are shown as MFI values (mean + SEM) and are representative of three independent experiments (triplicates each). Significance is indicated by asterisks, *=p<0.05; **=p<0.01; ***=p<0.001; ****=p<0.0001; ns = non-significant.(TIF)
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title: S1 Fig
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