Autoprocessing of neutrophil elastase near its active site reduces the 
efficiency of natural and synthetic elastase inhibitors

Dau, T.; Sarker, R. S. J.; Yildirim, A. O.; Eickelberg, O.; Jenne, D. E.

doi:10.1038/ncomms7722

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Autoprocessing of neutrophil elastase near its active site reduces the efficiency of natural and synthetic elastase inhibitors

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Jenne, D. E.
Research Group: Enzymes and Inhibitors in Chronic Lung Disease / Jenne, MPI of Neurobiology, Max Planck Society;

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引用

Dau, T., Sarker, R. S. J., Yildirim, A. O., Eickelberg, O., & Jenne, D. E. (2015). Autoprocessing of neutrophil elastase near its active site reduces the efficiency of natural and synthetic elastase inhibitors. Nature Communications, 6:. doi:10.1038/ncomms7722.

引用: https://hdl.handle.net/11858/00-001M-0000-0026-DC9E-5

要旨

An imbalance between neutrophil-derived proteases and extracellular inhibitors is widely regarded as an important pathogenic mechanism for lung injury. Despite intense efforts over the last three decades, attempts to develop small-molecule inhibitors for neutrophil elastase have failed in the clinic. Here we discover an intrinsic self-cleaving property of mouse neutrophil elastase that interferes with the action of elastase inhibitors. We show that conversion of the single-chain (sc) into a two-chain (tc) neutrophil elastase by self-cleavage near its S1 pocket altered substrate activity and impaired both inhibition by endogenous alpha-1-antitrypsin and synthetic small molecules. Our data indicate that autoconversion of neutrophil elastase decreases the inhibitory efficacy of natural alpha-1-antitrypsin and small-molecule inhibitors, while retaining its pathological potential in an experimental mouse model. The so-far overlooked occurrence and properties of a naturally occurring tc-form of neutrophil elastase necessitates the redesign of small-molecule inhibitors that target the sc-form as well as the tc-form of neutrophil elastase.