Miniature random-access fiber scanner for in vivo multiphoton imaging

Sawinski, Jürgen; Denk, Winfried

doi:10.1063/1.2763945

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Miniature random-access fiber scanner for in vivo multiphoton imaging

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Sawinski, Jürgen
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Denk, Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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http://dx.doi.org/10.1063/1.2763945
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Citation

Sawinski, J., & Denk, W. (2007). Miniature random-access fiber scanner for in vivo multiphoton imaging. Journal of Applied Physics, 102(3): 034701. doi:10.1063/1.2763945.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-9983-C

Abstract

Multiphoton microscopy allows imaging of cellular activity in living tissue explants and in whole animals and has thus become a powerful tool for studying neuronal activity. The miniaturization of laser−scanning microscopy promises to extend activity measurements to awake, freely moving animals. Here we describe and evaluate a miniature multiphoton microscope based on a "piezolever fiber scanner" (PLFS). Our current PLFS has a scan range of 1.1 mm and a resonance frequency of 790 Hz. It allows, unlike resonance−based designs, a lateral shift of the scanned area, and offers the prospect of random−access (vector) scanning