The three-dimensional micro- and nanostructure of the aortic medial lamellar 
unit measured using 3D confocal and electron microscopy imaging

O´Connell, Mary K.; Murthy, Sushila; Phan, Samson; Xu, Chengpei; Buchanan, JoAnn; Spilker, Ryan; Dalman, Ronald L.; Zarins, Christopher K.; Denk, Winfried; Taylor, Charles A.

doi:10.1016/j.matbio.2007.10.008

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学術論文

The three-dimensional micro- and nanostructure of the aortic medial lamellar unit measured using 3D confocal and electron microscopy imaging

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Denk, Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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http://www.sciencedirect.com/science/article/pii/S0945053X0700145X/pdfft?md5=affb6df864660f26e399cfa21e72dd5c&pid=1-s2.0-S0945053X0700145X-main.pdf
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http://dx.doi.org/10.1016/j.matbio.2007.10.008
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引用

O´Connell, M. K., Murthy, S., Phan, S., Xu, C., Buchanan, J., Spilker, R., Dalman, R. L., Zarins, C. K., Denk, W., & Taylor, C. A. (2008). The three-dimensional micro- and nanostructure of the aortic medial lamellar unit measured using 3D confocal and electron microscopy imaging. Matrix Biology, 27(3), 171-181. doi:10.1016/j.matbio.2007.10.008.

引用: https://hdl.handle.net/11858/00-001M-0000-0019-98BF-0

要旨

Changes in arterial wall composition and function underlie all forms of vascular disease. The fundamental structural and functional unit of the aortic
wall is the medial lamellar unit (MLU). While the basic composition and organization of the MLU is known, three−dimensional (3D) microstructural
details are tenuous, due (in part) to lack of three−dimensional data at micro− and nano−scales. We applied novel electron and confocal microscopy
techniques to obtain 3D volumetric information of aortic medial microstructure at micro− and nano−scales with all constituents present. For the rat
abdominal aorta, we show thatmedial elastin has three primary forms: with approximately 71%of total elastin as thick, continuous lamellar sheets, 27%
as thin, protruding interlamellar elastin fibers (IEFs), and 2%as thick radial struts. Elastin pores are not simply holes in lamellar sheets, but are indented
and gusseted openings in lamellae. Smooth muscle cells (SMCs) weave throughout the interlamellar elastin framework, with cytoplasmic extensions
abutting IEFs, resulting in approximately 20° radial tilt (relative to the lumen surface) of elliptical SMC nuclei. Collagen fibers are organized as large,
parallel bundles tightly envelopingSMC nuclei. Quantification of the orientation of collagen bundles, SMC nuclei, and IEFs reveal that all three primary
medial constituents have predominantly circumferential orientation, correlating with reported circumferentially dominant values of physiological stress,
collagen fiber recruitment, and tissue stiffness. This high resolution three−dimensional view of the aortic media reveals MLU microstructure details that
suggest a highly complex and integrated mural organization that correlates with aortic mechanical properties