Single−spike detection in vitro and in vivo with a genetic Ca2+ sensor

Wallace, Damian J; Meyer zum Alten Borgloh, Stephan; Astori, Simone; Yang, Ying; Bausen, Melanie; Kügler, Sebastian; Palmer, Amy E.; Tsien, Roger Y.; Sprengel, Rolf; Kerr, Jason ND; Denk, Winfried; Hasan, Mazahir T.

doi:10.1038/NMETH.1242

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Single−spike detection in vitro and in vivo with a genetic Ca2+ sensor

MPS-Authors

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Wallace, Damian J
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Meyer zum Alten Borgloh, Stephan
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Max Planck Research Group Behavioural Neurophysiology (Andreas T. Schaefer), Max Planck Institute for Medical Research, Max Planck Society;

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Astori, Simone
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Yang, Ying
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Bausen, Melanie
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Sprengel, Rolf
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Kerr, Jason ND
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Denk, Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Hasan, Mazahir T.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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http://dx.doi.org/10.1038/NMETH.1242
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http://www.nature.com/nmeth/journal/v5/n9/pdf/nmeth.1242.pdf
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引用

Wallace, D. J., Meyer zum Alten Borgloh, S., Astori, S., Yang, Y., Bausen, M., Kügler, S., Palmer, A. E., Tsien, R. Y., Sprengel, R., Kerr, J. N., Denk, W., & Hasan, M. T. (2008). Single−spike detection in vitro and in vivo with a genetic Ca2+ sensor. Nature Methods, 5(9), 797-804. doi:10.1038/NMETH.1242.

引用: https://hdl.handle.net/11858/00-001M-0000-0019-9839-D

要旨

Measurement of population activity with single−action−potential, single−neuron resolution is pivotal for understanding information representation and processing in the brain and how the brain's responses are altered by experience. Genetically encoded indicators of neuronal activity allow long−term, cell type−specific expression. Fluorescent Ca2+ indicator proteins (FCIPs), a main class of reporters of neural activity, initially suffered, in particular, from an inability to report single action potentials in vivo. Although suboptimal Ca2+−binding dynamics and Ca2+−induced fluorescence changes in FCIPs are important factors, low levels of expression also seem to play a role. Here we report that delivering D3cpv, an improved fluorescent resonance energy transfer−based FCIP, using a recombinant adeno−associated virus results in expression sufficient to detect the Ca2+ transients that accompany single action potentials. In upper−layer cortical neurons, we were able to detect transients associated with single action potentials firing at rates of <1 Hz, with high reliability, from in vivo recordings in living mice