Extended Data Figure 5: Positioning of the reactive nucleotides in the active centre of ligase deoxyribozyme 9DB1 in comparison to ribozymes.
From Crystal structure of a DNA catalyst
- Almudena Ponce-Salvatierra1, 2,
- Katarzyna Wawrzyniak-Turek1, 3,
- Ulrich Steuerwald2,
- Claudia Höbartner1, 3,
- Vladimir Pena2,
- Journal name:
- Nature
- Volume:
- 529,
- Pages:
- 231–234
- Date published:
- DOI:
- doi:10.1038/nature16471
Additional data
Affiliations
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Max Planck Research Group Nucleic Acid Chemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
- Almudena Ponce-Salvatierra,
- Katarzyna Wawrzyniak-Turek &
- Claudia Höbartner
-
Research Group Macromolecular Crystallography, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
- Almudena Ponce-Salvatierra,
- Ulrich Steuerwald &
- Vladimir Pena
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Institute for Organic and Biomolecular Chemistry, Georg-August-University Göttingen, Tammannstr. 2, 37077 Göttingen, Germany
- Katarzyna Wawrzyniak-Turek &
- Claudia Höbartner
Contributions
Crystallographic work was performed by A.P.-S. under the supervision of V.P., biochemical experiments were performed by A.P.-S., K.W.-T. and C.H. U.S. obtained initial crystals. A.P.-S., V.P. and C.H. designed the experiments and all authors discussed the results and contributed to the manuscript.
Competing financial interests
The authors declare no competing financial interests.
Structural models and structure factors have been deposited in the Protein Data Bank under accession numbers 5CKI (cobalt) and 5CKK (native).
Author details
Almudena Ponce-Salvatierra
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Katarzyna Wawrzyniak-Turek
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Ulrich Steuerwald
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Claudia Höbartner
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Vladimir Pena
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Extended Data Figure 1: Semi-continuous helix in the crystal lattice.Hover over figure to zoom
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Extended Data Figure 2: Influence of 2′-modifications on mutant acceptor RNA U-1.Hover over figure to zoom
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Extended Data Figure 3: Distribution of pseudorotation phase angels of 9DB1 deoxyribozyme in comparison with twister46 and L1 ligase ribozyme30.Hover over figure to zoom
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Extended Data Figure 4: Active sites of RNA-ligating nucleic acid enzymes.Hover over figure to zoom
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Extended Data Figure 5: Positioning of the reactive nucleotides in the active centre of ligase deoxyribozyme 9DB1 in comparison to ribozymes.Hover over figure to zoom
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Extended Data Table 1: Data collection and refinement statisticsHover over figure to zoom
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Extended Data Table 2: Oligonucleotides for kinetic experimentsHover over figure to zoom