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Journal Article

Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective

MPS-Authors
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Kerr,  Jason
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Nimmerjahn,  Axel
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons93373

Helmchen,  Fritjof
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://dx.doi.org/10.1364/OL.29.002521
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https://www.osapublishing.org/ol/viewmedia.cfm?uri=ol-29-21-2521&seq=0
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Citation

Göbel, W., Kerr, J., Nimmerjahn, A., & Helmchen, F. (2004). Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective. Optics Letters, 29(21), 2521-2523. doi:10.1364/OL.29.002521.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002A-18EF-6
Abstract
We present a miniature, flexible two-photon microscope consisting of a fused coherent optical fiber bundle with 30,000 cores and a gradient-index lens objective. The laser focus of a standard two-photon laser-scanning microscope was scanned over the entrance surface of the fiber bundle, resulting in sequential coupling into individual cores. Fluorescent light was detected through the fiber bundle. Micrometer-sized fluorescent beads and pollen grains were readily resolved. In addition, fluorescently labeled blood vessels were imaged through the fiber bundle in rat brain in vivo.