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prism.endingPage: 242
citation_title: Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes
WT.cg_s: Letter
access: Yes
keywords: Genomic instability, Meiosis, Chromosomes, High-throughput screening
emanating: 134X76X223X157
citation_publisher: Nature Publishing Group
citation_journal_title: Nature
description: During fertilization, an egg and a sperm fuse to form a new embryo. Eggs develop from oocytes in a process called meiosis. Meiosis in human oocytes is highly error-prone, and defective eggs are the leading cause of pregnancy loss and several genetic disorders such as Down/'s syndrome. Which genes safeguard accurate progression through meiosis is largely unclear. Here we develop high-content phenotypic screening methods for the systematic identification of mammalian meiotic genes. We targeted 774 genes by RNA interference within follicle-enclosed mouse oocytes to block protein expression from an early stage of oocyte development onwards. We then analysed the function of several genes simultaneously by high-resolution imaging of chromosomes and microtubules in live oocytes and scored each oocyte quantitatively for 50 phenotypes, generating a comprehensive resource of meiotic gene function. The screen generated an unprecedented annotated data set of meiotic progression in 2,241 mammalian oocytes, which allowed us to analyse systematically which defects are linked to abnormal chromosome segregation during meiosis, identifying progression into anaphase with misaligned chromosomes as well as defects in spindle organization as risk factors. This study demonstrates how high-content screens can be performed in oocytes, and allows systematic studies of meiosis in mammals.
WT.site_id: 18696
citation_date: 2015-08-13
title: ASPM is required to prevent lagging chromosomes in oocytes : Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes : Nature : Nature Publishing Group
DC.title: Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes
DC.date: 2015-07-06
prism.issn: 0028-0836
DC.publisher: Nature Publishing Group
citation_online_date: 2015-07-06
WT.cg_n: Nature
DC.creator: Sybille Pfender
prism.issue: 7564
DC.identifier: doi:10.1038/nature14568
dc:title: ASPM is required to prevent lagging chromosomes in oocytes : Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes : Nature : Nature Publishing Group
Content-Encoding: UTF-8
prism.publicationDate: 2015-07-06
robots: noarchive
DC.rights: © 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
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prism.rightsAgent: permissions@nature.com
citation_author: Sybille Pfender
citation_issue: 7564
prism.eIssn: 1476-4687
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DC.language: en
prism.volume: 524
citation_firstpage: 239
prism.startingPage: 239
WT.template: C
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prism.publicationName: Nature
citation_doi: doi:10.1038/nature14568
prism.section: Letter
citation_volume: 524
WT.site_id_name: Max Planck Inst for Biophysical Chemistry (Karl Friedrich Bonhoeffer Inst)
Content-Language: en
prism.copyright: © 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.