Supplementary Figure 8: BFA addition releases the network’s actin nucleators from vesicles.
From Vesicles modulate an actin network for asymmetric spindle positioning
- Journal name:
- Nature Cell Biology
- Volume:
- 15,
- Pages:
- 937–947
- Year published:
- DOI:
- doi:10.1038/ncb2802
- Received
- Accepted
- Published online
Additional data
Affiliations
-
Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
- Zuzana Holubcová,
- Gillian Howard &
- Melina Schuh
Contributions
G.H. analysed the localization of Spire2 by electron microscopy. Z.H. and M.S. designed and carried out all other experiments. M.S. and Z.H. wrote and edited the manuscript, respectively.
Competing financial interests
The authors declare no competing financial interests.
Author details
Zuzana Holubcová
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Gillian Howard
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Melina Schuh
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Supplementary Figure 1: Loss of Rab11a-positive vesicles leads to mislocalization of the vesicles’ cargo transferrin.Hover over figure to zoom
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Supplementary Figure 2: Rab11a-positive vesicles are dispensable for oocyte viability and progression through meiosis.Hover over figure to zoom
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Supplementary Figure 3: The spindle is trapped in a static actin network if the function of Rab11a-positive vesicles is blocked.Hover over figure to zoom
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Supplementary Figure 4: Network stabilization with jasplakinolide prevents asymmetric spindle positioning.Hover over figure to zoom
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Supplementary Figure 5: Myosin Vb mediates the actin network dynamics and asymmetric spindle positioning.Hover over figure to zoom
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Supplementary Figure 6: A moderate increase of network density does not block network dynamics or asymmetric spindle positioning.Hover over figure to zoom
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Supplementary Figure 7: Localization of the network’s actin nucleators in the presence and absence of Rab11-a positive vesicles.Hover over figure to zoom
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Supplementary Figure 8: BFA addition releases the network’s actin nucleators from vesicles.Hover over figure to zoom
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Video 1: Rab11a-positive vesicles driving actin network dynamics.
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Video 2: Rab11a-positive vesicles temporarily associate with the spindle during asymmetric spindle positioning.
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Video 3: The spindle is trapped in a static actin network if the function of Rab11a-positive vesicles is blocked.
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Video 4: Asymmetric spindle positioning requires Rab11a-positive vesicles.
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Video 5: 3D volume reconstruction of the trapped spindle shown in Supplementary Video S3.
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Video 6: Myosin Vb drives the actin network dynamics.
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Video 7: mEGFP-Spire2 is released from vesicles and the plasma membrane into the cytoplasm following BFA addition.