Supporting Information
Fluorogenic Probes for Multicolor Imaging in Living Cells
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Fluorogenic Probes for Multicolor Imaging in Living Cells
† Institute of Bioengineering, NCCR in Chemical
Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne
(EPFL), 1015 Lausanne, Switzerland
‡ Graduate
School of Pharmaceutical Sciences, The University
of Tokyo, 7-3-1, Hongo,
Bunkyo-ku, Tokyo 113-8654, Japan
§ Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
J. Am. Chem. Soc., 2016, 138 (30), pp 9365–9368
DOI: 10.1021/jacs.6b04782
Publication Date (Web): July 15, 2016
Copyright © 2016 American Chemical Society
Abstract
Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.
The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/jacs.6b04782.
