Carboxylated photoswitchable diarylethenes for biolabeling and super-resolution 
RESOLFT microscopy.

Roubinet, B.; Bossi, M. L.; Alt, P.; Leutenegger, M.; Shojaei, H.; Schnorrenberg, S.; Nizamov, S.; Irie, M.; Belov, V. N.; Hell, S. W.

doi:10.1002/anie.201607940

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Carboxylated photoswitchable diarylethenes for biolabeling and super-resolution RESOLFT microscopy.

MPS-Authors

/persons/resource/persons199835

Roubinet, B.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons14883

Bossi, M. L.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons199837

Alt, P.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15441

Leutenegger, M.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons204190

Shojaei, H.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons196937

Schnorrenberg, S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons118566

Nizamov, S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons14832

Belov, V. N.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15210

Hell, S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

2354743.pdf
(出版社版), 3MB

付随資料 (公開)

2354743_Suppl.pdf
(付録資料), 5MB

引用

Roubinet, B., Bossi, M. L., Alt, P., Leutenegger, M., Shojaei, H., Schnorrenberg, S., Nizamov, S., Irie, M., Belov, V. N., & Hell, S. W. (2016). Carboxylated photoswitchable diarylethenes for biolabeling and super-resolution RESOLFT microscopy. Angewandte Chemie International Edition, 55(49), 15429-15433. doi:10.1002/anie.201607940.

引用: https://hdl.handle.net/11858/00-001M-0000-002B-AC7B-5

要旨

Reversibly photoswitchable 1,2-bis(2-ethyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes (EBT) having fluorescent "closed" forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology-related super-resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm.