Structure of a transcribing RNA polymerase II-DSIF complex reveals a 
multidentate DNA-RNA clamp.

Bernecky, C.; Plitzko, J. M.; Cramer, P.

doi:10.1038/nsmb.3465

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp.

MPS-Authors

/persons/resource/persons188847

Bernecky, C.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons127020

Cramer, P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

2477044.pdf
(全文テキスト（全般）), 170KB

付随資料 (公開)

2477044_Suppl_1.pdf
(付録資料), 2MB

2477044_Suppl_2.pdf
(付録資料), 139KB

2477044_Suppl_3.mp4
(付録資料), 21MB

引用

Bernecky, C., Plitzko, J. M., & Cramer, P. (2017). Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp. Nature Structural and Molecular Biology, 24(10), 809-815. doi:10.1038/nsmb.3465.

引用: https://hdl.handle.net/11858/00-001M-0000-002D-E222-4

要旨

During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation complex at a nominal resolution of 3.4 Å. Human DSIF has a modular structure with two domains forming a DNA clamp, two domains forming an RNA clamp, and one domain buttressing the RNA clamp. The clamps maintain the transcription bubble, position upstream DNA, and retain the RNA transcript in the exit tunnel. The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. The structure provides insight into the roles of DSIF during mRNA synthesis.