Supplementary Figure 2: Cryo-EM reconstruction statistics.
From Structure of a transcribing RNA polymerase II–DSIF complex reveals a multidentate DNA–RNA clamp
- Journal name:
- Nature Structural & Molecular Biology
- Year published:
- DOI:
- doi:10.1038/nsmb.3465
- Received
- Accepted
- Published online
Additional data
Affiliations
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Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
- Carrie Bernecky &
- Patrick Cramer
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Department of Molecular Structural Biology, Max Planck Institute for Biochemistry, Martinsried, Germany.
- Jürgen M Plitzko
Contributions
C.B. designed and carried out experiments and data analysis. J.M.P. provided access to a high-end EM facility and provided advice on microscope setup. P.C. supervised research. C.B. and P.C. prepared the manuscript.
Competing financial interests
The authors declare no competing financial interests.
Author details
Carrie Bernecky
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Jürgen M Plitzko
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Patrick Cramer
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Supplementary Figure 1: Cryo-EM analysis of the Pol II–DSIF EC.Hover over figure to zoom
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Supplementary Figure 2: Cryo-EM reconstruction statistics.Hover over figure to zoom
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Supplementary Figure 3: Cryo-EM and X-ray modeling and density fit of the Pol II–DSIF EC and the KOW6–7 domain.Hover over figure to zoom
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Supplementary Figure 4: Details of the DSIF DNA clamp.Hover over figure to zoom
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Supplementary Figure 5: Mutations in DSIF regions that contact nucleic acids are important for the binding of DSIF to the Pol II EC.Hover over figure to zoom
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Video 1: Three-dimensional view of the Pol II-DSIF EC.