Major satellite repeat RNA stabilize heterochromatin retention of Suv39h 
enzymes by RNA-nucleosome assocation and RNA:DNA hybrid formation

Camacho, Oscar Velazquez; Galan, Carmen; Swist-Rosowska, Kalina; Ching, Reagan; Gamalinda, Michael; Karabiber, Fethullah; De la Rosa-Velazquez, Inti Alberto; Engist, Bettina; Koschorz, Birgit; Shukeir, Nicholas; Onishi-Seebacher, Megumi; van de Nobelen, Suzanne; Jenuwein, Thomas

doi:10.7554/eLife.25293

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学術論文

Major satellite repeat RNA stabilize heterochromatin retention of Suv39h enzymes by RNA-nucleosome assocation and RNA:DNA hybrid formation

MPS-Authors

Camacho, Oscar Velazquez
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Galan, Carmen
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Swist-Rosowska, Kalina
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Ching, Reagan
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Gamalinda, Michael
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons191021

De la Rosa-Velazquez, Inti Alberto
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Engist, Bettina
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Koschorz, Birgit
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons256260

Shukeir, Nicholas
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Onishi-Seebacher, Megumi
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

van de Nobelen, Suzanne
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons191130

Jenuwein, Thomas
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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https://elifesciences.org/articles/25293
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引用

Camacho, O. V., Galan, C., Swist-Rosowska, K., Ching, R., Gamalinda, M., Karabiber, F., De la Rosa-Velazquez, I. A., Engist, B., Koschorz, B., Shukeir, N., Onishi-Seebacher, M., van de Nobelen, S., & Jenuwein, T. (2017). Major satellite repeat RNA stabilize heterochromatin retention of Suv39h enzymes by RNA-nucleosome assocation and RNA:DNA hybrid formation. eLife, e25293. doi:10.7554/eLife.25293.

引用: https://hdl.handle.net/21.11116/0000-0000-BFCF-1

要旨

The Suv39h1 and Suv39h2 histone lysine methyltransferases are hallmark enzymes at mammalian heterochromatin. We show here that the mouse Suv39h2 enzyme differs from Suv39h1 by containing an N-terminal basic domain that facilitates retention at mitotic chromatin and provides an additional affinity for major satellite repeat RNA. To analyze an RNA-dependent interaction with chromatin, we purified native nucleosomes from mouse ES cells and detect that Suv39h1 and Suv39h2 exclusively associate with poly-nucleosomes. This association was attenuated upon RNaseH incubation and entirely lost upon RNaseA digestion of native chromatin. Major satellite repeat transcripts remain chromatin-associated and have a secondary structure that favors RNA:DNA hybrid formation. Together, these data reveal an RNA-mediated mechanism for the stable chromatin interaction of the Suv39h KMT and suggest a function for major satellite non-coding RNA in the organization of an RNA-nucleosome scaffold as the underlying structure of mouse heterochromatin.