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Expansion microscopy of zebrafish for neuroscience and developmental biology studies
Limor Freifeld, Iris Odstrcil, Dominique Förster, Alyson Ramirez, James A. Gagnon, Owen Randlett, Emma K. Costa, Shoh Asano, Orhan T. Celiker, Ruixuan Gao, Daniel A. Martin-Alarcon, Paul Reginato, Cortni Dick, Linlin Chen, David Schoppik, Florian Engert, Herwig Baier and Edward S. Boyden
PNAS November 21, 2017. 201706281; published ahead of print November 21, 2017. https://doi.org/10.1073/pnas.1706281114
Limor Freifeld
aMedia Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
Iris Odstrcil
bDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
Dominique Förster
cDepartment Genes–Circuits–Behavior, Max Planck Institute of Neurobiology, Martinsried 82152, Germany;
Alyson Ramirez
bDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
James A. Gagnon
bDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
Owen Randlett
bDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
Emma K. Costa
dDepartment of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139;
Shoh Asano
aMedia Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
Orhan T. Celiker
eDepartment of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139;
Ruixuan Gao
aMedia Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;fMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139;
Daniel A. Martin-Alarcon
gDepartment of Biological Engineering, MIT, Cambridge, MA 02139;
Paul Reginato
gDepartment of Biological Engineering, MIT, Cambridge, MA 02139;hDepartment of Genetics, Harvard Medical School, Cambridge, MA 02138;
Cortni Dick
aMedia Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;
Linlin Chen
aMedia Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;iNeuroscience Program, Wellesley College, Wellesley, MA 02481;
David Schoppik
jDepartment of Otolaryngology, New York University School of Medicine, New York, NY 10016;kDepartment of Neuroscience and Physiology, New York University School of Medicine, New York, NY 10016;lNeuroscience Institute, New York University School of Medicine, New York NY 10016;
Florian Engert
bDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138;
Herwig Baier
cDepartment Genes–Circuits–Behavior, Max Planck Institute of Neurobiology, Martinsried 82152, Germany;
Edward S. Boyden
aMedia Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;dDepartment of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139;eDepartment of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139;fMcGovern Institute for Brain Research, MIT, Cambridge, MA 02139;mCenter for Neurobiological Engineering, MIT, Cambridge, MA 02139
Supporting Information
- Download Supporting Information (PDF)
- Download Appendix (PDF)
- Download Movie_S01 (MOV) - Three-dimensional rendering of the volume surrounding the radial glia cell RG1 showing this cell and its processes and, subsequently, an overlay of its tracing.
- Download Movie_S02 (AVI) - Run through a stack containing the nIII nucleus, with GFP-labeled cell bodies (yellow) and Kaede-labeled projections (magenta) in the expanded 6-d postfertilization larval zebrafish brain stained with anti–pan-MAGUK (cyan) shown in Fig. 2B.
- Download Movie_S03 (AVI) - Run through a stack containing the M cell axon-cap area containing spiral fiber neurons (magenta) in the expanded 6-d postfertilization larval zebrafish brain stained with anti-synaptotagmin2b (cyan) shown in Fig. 3C, Top.
- Download Movie_S04 (AVI) - Three-dimensional rendering of the M cell body and axon-cap area in the expanded 6-d postfertilization larval zebrafish brain stained with anti-glycine receptor antibody (gray) shown in Fig. 3D, Right.
- Download Movie_S05 (AVI) - Run through a stack containing the nucleus shown in Fig. 4B from an expanded shield-stage zebrafish embryo.
- Download Movie_S06 (AVI) - Run through the stack containing the nucleus shown in the top row of Fig. S10 from an expanded shield-stage zebrafish embryo.
- Download Movie_S07 (AVI) - Run through the stack containing the nucleus shown in Fig. 5A from an expanded shield-stage zebrafish embryo.
- Download Movie_S08 (AVI) - Run through the stack containing the nucleus shown in Fig. 5C from an expanded shield-stage zebrafish embryo.