Clonal expansion capacity defines two consecutive developmental stages of 
long-term hematopoietic stem cells.

Grinenko, Tatyana; Arndt, Kathrin; Portz, Melanie; Mende, Nicole; Günther, Marko; Cosgun, Kadriye Nehir; Alexopoulou, Dimitra; Lakshmanaperumal, Naharajan; Henry, Ian; Dahl, Andreas; Waskow, Claudia

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Clonal expansion capacity defines two consecutive developmental stages of long-term hematopoietic stem cells.

MPS-Authors

Portz, Melanie
Max Planck Society;

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Lakshmanaperumal, Naharajan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Henry, Ian
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Dahl, Andreas
Max Planck Society;

Waskow, Claudia
Max Planck Society;

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引用

Grinenko, T., Arndt, K., Portz, M., Mende, N., Günther, M., Cosgun, K. N., Alexopoulou, D., Lakshmanaperumal, N., Henry, I., Dahl, A., & Waskow, C. (2014). Clonal expansion capacity defines two consecutive developmental stages of long-term hematopoietic stem cells. The Journal of Experimental Medicine, 211(2), 209-215.

引用: https://hdl.handle.net/21.11116/0000-0001-0550-F

要旨

Long-term hematopoietic stem cells (HSCs [LT-HSCs]) are well known to display unpredictable differences in their clonal expansion capacities after transplantation. Here, by analyzing the cellular output after transplantation of stem cells differing in surface expression levels of the Kit receptor, we show that LT-HSCs can be systematically subdivided into two subtypes with distinct reconstitution behavior. LT-HSCs expressing intermediate levels of Kit receptor (Kit(int)) are quiescent in situ but proliferate extensively after transplantation and therefore repopulate large parts of the recipient's hematopoietic system. In contrast, metabolically active Kit(hi) LT-HSCs display more limited expansion capacities and show reduced but robust levels of repopulation after transfer. Transplantation into secondary and tertiary recipient mice show maintenance of efficient repopulation capacities of Kit(int) but not of Kit(hi) LT-HSCs. Initiation of differentiation is marked by the transit from Kit(int) to Kit(hi) HSCs, both of which precede any other known stem cell population.