A genomic toolkit to investigate kinesin and myosin motor function in cells.

Maliga, Zoltan; Junqueira, Magno; Toyoda, Yusuke; Ettinger, Andreas; Mora-Bermúdez, Felipe; Klemm, Robin; Vasilj, Andrej; Guhr, E.; Ibarlucea-Benitez, Itziar; Poser, Ina; Bonifacio, Enzio; Huttner, Wieland B.; Shevchenko, Andrej; Hyman, Anthony

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A genomic toolkit to investigate kinesin and myosin motor function in cells.

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Maliga, Zoltan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219281

Junqueira, Magno
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219747

Toyoda, Yusuke
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219145

Ettinger, Andreas
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219463

Mora-Bermúdez, Felipe
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219318

Klemm, Robin
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219758

Vasilj, Andrej
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219210

Guhr, E.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219545

Poser, Ina
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219023

Bonifacio, Enzio
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219252

Huttner, Wieland B.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons218972

Shevchenko, Andrej
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219253

Hyman, Anthony
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Maliga, Z., Junqueira, M., Toyoda, Y., Ettinger, A., Mora-Bermúdez, F., Klemm, R., et al. (2013). A genomic toolkit to investigate kinesin and myosin motor function in cells. Nature Cell Biology, 15(3), 325-334.

Cite as: https://hdl.handle.net/21.11116/0000-0001-067C-E

Abstract

Coordination of multiple kinesin and myosin motors is required for intracellular transport, cell motility and mitosis. However, comprehensive resources that allow systems analysis of the localization and interplay between motors in living cells do not exist. Here, we generated a library of 243 amino- and carboxy-terminally tagged mouse and human bacterial artificial chromosome transgenes to establish 227 stably transfected HeLa cell lines, 15 mouse embryonic stem cell lines and 1 transgenic mouse line. The cells were characterized by expression and localization analyses and further investigated by affinity-purification mass spectrometry, identifying 191 candidate protein-protein interactions. We illustrate the power of this resource in two ways. First, by characterizing a network of interactions that targets CEP170 to centrosomes, and second, by showing that kinesin light-chain heterodimers bind conventional kinesin in cells. Our work provides a set of validated resources and candidate molecular pathways to investigate motor protein function across cell lineages.