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Systematic analysis of human protein complexes identifies chromosome segregation proteins.

MPG-Autoren
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Toyoda,  Yusuke
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Poser,  Ina
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Hériché,  Jean-Karim
Max Planck Society;

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Augsburg,  Martina
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Sarov,  Mihail
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Pozniakovsky,  Andrei I.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Slabicki,  Mikolaj
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Ssykor,  Andrea
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Pelletier,  Laurence
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Buchholz,  Frank
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Hyman,  Anthony A.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Peters,  Jan-Michael
Max Planck Society;

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Zitation

Hutchins, J. R. A., Toyoda, Y., Hegemann, B., Poser, I., Hériché, J.-K., Sykora, M. M., et al. (2010). Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.), 328(5978), 593-599.


Zitierlink: https://hdl.handle.net/21.11116/0000-0001-0C35-7
Zusammenfassung
Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate that the identity and composition of these protein complexes is incompletely understood. Using gene tagging on bacterial artificial chromosomes, protein localization, and tandem-affinity purification-mass spectrometry, the MitoCheck consortium has analyzed about 100 human protein complexes, many of which had not or had only incompletely been characterized. This work has led to the discovery of previously unknown, evolutionarily conserved subunits of the anaphase-promoting complex and the gamma-tubulin ring complex--large complexes that are essential for spindle assembly and chromosome segregation. The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells.