Fluorogenic probes for live-cell imaging of the cytoskeleton.

Lukinavicius, G.; Reymond, L.; D'Este, E.; Masharina, A.; Göttfert, F.; Ta, H.; Guether, A.; Fournier, M.; Rizzo, S.; Waldmann, H.; Blaukopf, C.; Sommer, C.; Gerlich, D. W.; Arndt, H. D.; Hell, S. W.; Johnsson, K.

doi:10.1038/nmeth.2972

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Fluorogenic probes for live-cell imaging of the cytoskeleton.

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Lukinavicius, G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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D'Este, E.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Göttfert, F.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Ta, H.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Hell, S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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2616375.pdf
(出版社版), 637KB

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2616375_Suppl.2972
(付録資料), 245KB

引用

Lukinavicius, G., Reymond, L., D'Este, E., Masharina, A., Göttfert, F., Ta, H., Guether, A., Fournier, M., Rizzo, S., Waldmann, H., Blaukopf, C., Sommer, C., Gerlich, D. W., Arndt, H. D., Hell, S. W., & Johnsson, K. (2014). Fluorogenic probes for live-cell imaging of the cytoskeleton. Nature Methods, 11(7), 731-733. doi:10.1038/nmeth.2972.

引用: https://hdl.handle.net/21.11116/0000-0001-A5DC-D

要旨

We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.