Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Awel, S.; Kirian, R. A.; Wiedorn, M. O.; Beyerlein, K. R.; Roth, N.; Horke, D. A.; Oberthür, D.; Knoska, J.; Mariani, V.; Morgan, A.; Adriano, L.; Tolstikova, A.; Paulraj, L. X.; Yefanov, O.; Aquila, A.; Barty, A.; Roy-Chowdhury, S.; Hunter, M. S.; James, D.; Robinson, J. S.; Weierstall, U.; Rode, A. V.; Bajt, S.; Küpper, J.; Chapman, H. N.

doi:10.1107/S1600576717018131

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Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

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Paulraj, L. X.
Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY;
International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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https://dx.doi.org/10.1107/S1600576717018131
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引用

Awel, S., Kirian, R. A., Wiedorn, M. O., Beyerlein, K. R., Roth, N., Horke, D. A., Oberthür, D., Knoska, J., Mariani, V., Morgan, A., Adriano, L., Tolstikova, A., Paulraj, L. X., Yefanov, O., Aquila, A., Barty, A., Roy-Chowdhury, S., Hunter, M. S., James, D., Robinson, J. S., Weierstall, U., Rode, A. V., Bajt, S., Küpper, J., & Chapman, H. N. (2018). Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. Journal of Applied Crystallography, 51, 133-139. doi:10.1107/S1600576717018131.

引用: https://hdl.handle.net/21.11116/0000-0001-D60C-1

要旨

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.