Fundamental parameters of the developing thymic epithelium in the mouse

Hirakawa, Mayumi; Nagakubo, Daisuke; Kanzler, Benoit; Avilov, Sergiy; Krauth, Brigitte; Happe, Christiane; Swann, Jeremy B.; Nusser, Anja; Boehm, Thomas

doi:10.1038/s41598-018-29460-0

Item

ITEM ACTIONSEXPORT

Add to Basket

Please note that a newer version of this item is available:
https://pure.mpg.de/pubman/item/item_3008666_5

DetailsSummary

Released

Journal Article

Fundamental parameters of the developing thymic epithelium in the mouse

MPS-Authors

Hirakawa, Mayumi
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Nagakubo, Daisuke
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Kanzler, Benoit
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons241905

Avilov, Sergiy
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Krauth, Brigitte
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Happe, Christiane
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Swann, Jeremy B.
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Nusser, Anja
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons190993

Boehm, Thomas
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

External Resource

https://www.nature.com/articles/s41598-018-29460-0.pdf
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Hirakawa, M., Nagakubo, D., Kanzler, B., Avilov, S., Krauth, B., Happe, C., et al. (2018). Fundamental parameters of the developing thymic epithelium in the mouse. Scientific Reports, 8, 11095. doi:10.1038/s41598-018-29460-0.

Cite as: https://hdl.handle.net/21.11116/0000-0002-C2B9-2

Abstract

The numbers of thymic epithelial cells (TECs) and thymocytes steadily increase during embryogenesis. To examine this dynamic, we generated several TEC-specific transgenic mouse lines, which express fluorescent proteins in the nucleus, the cytosol and in the membranes under the control of the Foxn1 promoter. These tools enabled us to determine TEC numbers in tissue sections by confocal fluorescent microscopy, and in the intact organ by light-sheet microscopy. Compared to histological procedures, flow cytometric analysis of thymic cellularity is shown to underestimate the numbers of TECs by one order of magnitude; using enzymatic digestion of thymic tissue, the loss of cortical TECs (cTECs) is several fold greater than that of medullary TECs (mTECs), although different cTEC subsets appear to be still present in the final preparation. Novel reporter lines driven by Psmb11 and Prss16 promoters revealed the trajectory of differentiation of cTEC-like cells, and, owing to the additional facility of conditional cell ablation, allowed us to follow the recovery of such cells after their depletion during embryogenesis. Multiparametric histological analyses indicate that the new transgenic reporter lines not only reveal the unique morphologies of different TEC subsets, but are also conducive to the analysis of the complex cellular interactions in the thymus.