date: 2018-10-31T15:30:40Z
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pdf:docinfo:title: Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization
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dc:description: This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged.
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description: This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged.
dcterms:created: 2018-09-13T12:00:00Z
Last-Modified: 2018-10-31T15:30:40Z
dcterms:modified: 2018-10-31T15:30:40Z
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title: Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization
Last-Save-Date: 2018-10-31T15:30:40Z
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pdf:docinfo:modified: 2018-10-31T15:30:40Z
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dc:title: Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization
modified: 2018-10-31T15:30:40Z
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meta:creation-date: 2018-09-13T12:00:00Z
created: 2018-09-13T12:00:00Z
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Creation-Date: 2018-09-13T12:00:00Z
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producer: International Union of Crystallography
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pdf:docinfo:producer: International Union of Crystallography
pdf:docinfo:created: 2018-09-13T12:00:00Z