date: 2017-06-03T07:08:52Z
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pdf:docinfo:title: Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations
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subject: Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at -80 C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations.
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dc:title: Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations
modified: 2017-06-03T07:08:52Z
cp:subject: Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at -80 C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations.
pdf:docinfo:subject: Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at -80 C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations.
pdf:docinfo:creator: Maryna Polyakova, Haiko Schlögl, Julia Sacher, Maren Schmidt-Kassow, Jochen Kaiser, Michael Stumvoll, Jürgen Kratzsch and Matthias L. Schroeter
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meta:author: Maryna Polyakova, Haiko Schlögl, Julia Sacher, Maren Schmidt-Kassow, Jochen Kaiser, Michael Stumvoll, Jürgen Kratzsch and Matthias L. Schroeter
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Author: Maryna Polyakova, Haiko Schlögl, Julia Sacher, Maren Schmidt-Kassow, Jochen Kaiser, Michael Stumvoll, Jürgen Kratzsch and Matthias L. Schroeter
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Keywords: BDNF; brain-derived neurotrophic factor; serum; EDTA-plasma; stability; inter-assay precision; acceptable change limit
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dc:creator: Maryna Polyakova, Haiko Schlögl, Julia Sacher, Maren Schmidt-Kassow, Jochen Kaiser, Michael Stumvoll, Jürgen Kratzsch and Matthias L. Schroeter
dcterms:created: 2017-06-03T07:08:52Z
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title: Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations
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pdf:docinfo:keywords: BDNF; brain-derived neurotrophic factor; serum; EDTA-plasma; stability; inter-assay precision; acceptable change limit
pdf:docinfo:modified: 2017-06-03T07:08:52Z
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creator: Maryna Polyakova, Haiko Schlögl, Julia Sacher, Maren Schmidt-Kassow, Jochen Kaiser, Michael Stumvoll, Jürgen Kratzsch and Matthias L. Schroeter
dc:subject: BDNF; brain-derived neurotrophic factor; serum; EDTA-plasma; stability; inter-assay precision; acceptable change limit
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meta:keyword: BDNF; brain-derived neurotrophic factor; serum; EDTA-plasma; stability; inter-assay precision; acceptable change limit
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pdf:docinfo:created: 2017-06-03T07:08:52Z