date: 2019-03-12T12:44:33Z
pdf:unmappedUnicodeCharsPerPage: 7
pdf:PDFVersion: 1.5
pdf:docinfo:title: Fixed-target serial oscillation crystallography at room temperature
xmp:CreatorTool: pdftk 3.0.0 - www.pdftk.com
dc:description: A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1?5° of data per crystal at room temperature with fast (10°?s?1) oscillation rates and translation times, giving a crystal-data collection rate of 2.5?Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement ? up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal.
access_permission:modify_annotations: true
access_permission:can_print_degraded: true
description: A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1?5° of data per crystal at room temperature with fast (10°?s?1) oscillation rates and translation times, giving a crystal-data collection rate of 2.5?Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement ? up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal.
dcterms:created: 2019-02-23T12:00:00Z
Last-Modified: 2019-03-12T12:44:33Z
dcterms:modified: 2019-03-12T12:44:33Z
dc:format: application/pdf; version=1.5
title: Fixed-target serial oscillation crystallography at room temperature
Last-Save-Date: 2019-03-12T12:44:33Z
pdf:docinfo:creator_tool: pdftk 3.0.0 - www.pdftk.com
access_permission:fill_in_form: true
pdf:docinfo:modified: 2019-03-12T12:44:33Z
meta:save-date: 2019-03-12T12:44:33Z
pdf:encrypted: false
dc:title: Fixed-target serial oscillation crystallography at room temperature
modified: 2019-03-12T12:44:33Z
Content-Type: application/pdf
X-Parsed-By: org.apache.tika.parser.DefaultParser
meta:creation-date: 2019-02-23T12:00:00Z
created: 2019-02-23T12:00:00Z
access_permission:extract_for_accessibility: true
access_permission:assemble_document: true
xmpTPg:NPages: 12
Creation-Date: 2019-02-23T12:00:00Z
pdf:charsPerPage: 3536
access_permission:extract_content: true
access_permission:can_print: true
producer: International Union of Crystallography
access_permission:can_modify: true
pdf:docinfo:producer: International Union of Crystallography
pdf:docinfo:created: 2019-02-23T12:00:00Z