Programmable functionalization of surfactant‐stabilized microfluidic droplets 
via DNA‐tags

Jahnke, Kevin; Weiss, Marian; Frey, Christoph; Antona, Silvia; Janiesch, Jan-Willi; Platzman, Ilia; Göpfrich, Kerstin; Spatz, Joachim P.

doi:10.1002/adfm.201808647

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学術論文

Programmable functionalization of surfactant‐stabilized microfluidic droplets via DNA‐tags

MPS-Authors

/persons/resource/persons232747

Jahnke, Kevin
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons140010

Weiss, Marian
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons201484

Frey, Christoph
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons232754

Antona, Silvia
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons75624

Janiesch, Jan-Willi
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons84351

Platzman, Ilia
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons220391

Göpfrich, Kerstin
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons76135

Spatz, Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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引用

Jahnke, K., Weiss, M., Frey, C., Antona, S., Janiesch, J.-W., Platzman, I., Göpfrich, K., & Spatz, J. P. (2019). Programmable functionalization of surfactant‐stabilized microfluidic droplets via DNA‐tags. Advanced Functional Materials, 29(23):, pp. 1-8. doi:10.1002/adfm.201808647.

引用: https://hdl.handle.net/21.11116/0000-0003-736E-1

要旨

Droplet‐based microfluidics has emerged as a powerful tool in synthetic biology. For many applications, chemical functionalization of the droplets is a key process. Therefore, a straightforward and broadly applicable approach is developed to functionalize the inner periphery of microfluidic droplets with diverse reactive groups and components. Instead of covalent modification of the droplet‐stabilizing surfactants, this method relies on cholesterol‐tagged DNA that self‐assembles at the droplet periphery. The cholesterol‐tagged DNA serves as an attachment handle for the recruitment of complementary DNA. The complementary DNA can carry diverse functional groups. We exemplify our method by demonstrating the attachment of amine groups, DNA nanostructures, microspheres, a minimal actin cortex, and leukemia cells to the droplet periphery. It is further shown that the DNA‐mediated attachment to the droplet periphery is temperature‐responsive and reversible. It is envisioned that droplet functionalization via DNA handles will help to tailor droplet interfaces for diverse applications—featuring programmable assembly, unique addressability, and stimuli‐responsiveness.