dc.publisher: Multidisciplinary Digital Publishing Institute
og:image: https://www.mdpi.com/sensors/sensors-19-02065/article_deploy/html/images/sensors-19-02065-ag-550.jpg
twitter:card: summary
og:site_name: MDPI
citation_journal_title: Sensors
dc.rights: http://creativecommons.org/licenses/by/3.0/
og:description: Point-of-care systems enable fast therapy decisions on site without the need of any healthcare infrastructure. In addition to the sensitive detection, stable measurement by inexperienced persons outside of laboratory facilities is indispensable. A particular challenge in field applications is to reduce interference from environmental factors, such as temperature, to acceptable levels without sacrificing simplicity. Here, we present a smartphone-based point-of-care sensor. The method uses an optofluidic grating composed of alternating detection and reference channels arranged as a reflective phase grating. Biomolecules adsorbing to the detection channel alter the optical path length, while the parallel reference channels enable a direct common mode rejection within a single measurement. The optical setup is integrated in a compact design of a mobile readout device and the usability is ensured by a smartphone application. Our results show that different ambient temperatures do not have any influence on the signal. In a proof-of concept experiment we measured the accumulation of specific molecules in functionalized detection channels in real-time and without the need of any labeling. Therefore, the channel walls have been modified with biotin as capture molecules and the specific binding of streptavidin was detected. A mobile, reliable and robust point-of-care device has been realized by combining an inherently differential measurement concept with a smartphone-based, mobile readout device.
prism.number: 9
dc:title: Sensors | Free Full-Text | Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry
Content-Encoding: UTF-8
citation_pdf_url: https://www.mdpi.com/1424-8220/19/9/2065/pdf
application-name:  
google-site-verification: PxTlsg7z2S00aHroktQd57fxygEjMiNHydKn3txhvwY
citation_author: Purr, Foelke
dc.date: 2019-05-03
citation_abstract_html_url: https://www.mdpi.com/1424-8220/19/9/2065
citation_issue: 9
fulltext_html: https://www.mdpi.com/1424-8220/19/9/2065/htm
prism.volume: 19
fulltext_pdf: https://www.mdpi.com/1424-8220/19/9/2065/pdf
prism.publicationName: Sensors
citation_doi: 10.3390/s19092065
dc.title: Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry
citation_volume: 19
dc.language: en
Content-Language: en
msapplication-TileImage: /img/journals/sensors-logo-sq.png
citation_publication_date: 2019/1
theme-color: #ffffff
citation_title: Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry
citation_publisher: Multidisciplinary Digital Publishing Institute
citation_id: mdpi-s19092065
dc.format: application/pdf
description: Point-of-care systems enable fast therapy decisions on site without the need of any healthcare infrastructure. In addition to the sensitive detection, stable measurement by inexperienced persons outside of laboratory facilities is indispensable. A particular challenge in field applications is to reduce interference from environmental factors, such as temperature, to acceptable levels without sacrificing simplicity. Here, we present a smartphone-based point-of-care sensor. The method uses an optofluidic grating composed of alternating detection and reference channels arranged as a reflective phase grating. Biomolecules adsorbing to the detection channel alter the optical path length, while the parallel reference channels enable a direct common mode rejection within a single measurement. The optical setup is integrated in a compact design of a mobile readout device and the usability is ensured by a smartphone application. Our results show that different ambient temperatures do not have any influence on the signal. In a proof-of concept experiment we measured the accumulation of specific molecules in functionalized detection channels in real-time and without the need of any labeling. Therefore, the channel walls have been modified with biotin as capture molecules and the specific binding of streptavidin was detected. A mobile, reliable and robust point-of-care device has been realized by combining an inherently differential measurement concept with a smartphone-based, mobile readout device.
title: Sensors | Free Full-Text | Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry
twitter:image: https://www.mdpi.com/img/journals/sensors-logo-sq.png
citation_online_date: 2019/05/03
fulltext_xml: https://www.mdpi.com/1424-8220/19/9/2065/xml
twitter:site: @MDPIOpenAccess
dc.type: Article
dc.source: Sensors 2019, Vol. 19, Page 2065
dc.creator: Foelke Purr
citation_fulltext_html_url: https://www.mdpi.com/1424-8220/19/9/2065/htm
prism.publicationDate: 2019-05-03
Content-Type: text/html; charset=UTF-8
X-Parsed-By: org.apache.tika.parser.DefaultParser
dc.description: Point-of-care systems enable fast therapy decisions on site without the need of any healthcare infrastructure. In addition to the sensitive detection, stable measurement by inexperienced persons outside of laboratory facilities is indispensable. A particular challenge in field applications is to reduce interference from environmental factors, such as temperature, to acceptable levels without sacrificing simplicity. Here, we present a smartphone-based point-of-care sensor. The method uses an optofluidic grating composed of alternating detection and reference channels arranged as a reflective phase grating. Biomolecules adsorbing to the detection channel alter the optical path length, while the parallel reference channels enable a direct common mode rejection within a single measurement. The optical setup is integrated in a compact design of a mobile readout device and the usability is ensured by a smartphone application. Our results show that different ambient temperatures do not have any influence on the signal. In a proof-of concept experiment we measured the accumulation of specific molecules in functionalized detection channels in real-time and without the need of any labeling. Therefore, the channel walls have been modified with biotin as capture molecules and the specific binding of streptavidin was detected. A mobile, reliable and robust point-of-care device has been realized by combining an inherently differential measurement concept with a smartphone-based, mobile readout device.
og:type: article
og:title: Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry
citation_authors: Purr, Foelke ; Eckardt, Max-Frederik ; Kieserling, Jonas ; Gronwald, Paul-Luis ; Burg, Thomas  P.; Dietzel, Andreas 
X-UA-Compatible: IE=edge,chrome=1
citation_firstpage: 2065
fb:app_id: 131189377574
prism.startingPage: 2065
viewport: width=device-width, initial-scale=1.0
dc.identifier: 10.3390/s19092065
dc.subject: biosensing
prism.section: Article
og:url: https://www.mdpi.com/1424-8220/19/9/2065