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Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system
View ORCID ProfileCarola Gregor, View ORCID ProfileJasmin K. Pape, View ORCID ProfileKlaus C. Gwosch, Tanja Gilat, View ORCID ProfileSteffen J. Sahl, and View ORCID ProfileStefan W. Hell
Carola Gregor
aDepartment of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany;
Jasmin K. Pape
aDepartment of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany;
Klaus C. Gwosch
aDepartment of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany;
Tanja Gilat
aDepartment of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany;
Steffen J. Sahl
aDepartment of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany;
Stefan W. Hell
aDepartment of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany;bDepartment of Optical Nanoscopy, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany
Contributed by Stefan W. Hell, November 1, 2019 (sent for review August 6, 2019; reviewed by Peter Macheroux and Alice Y. Ting)
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- Download Appendix (PDF)
- Download Movie_S01 (MOV) - Cell division of HeLa cells expressing co lux. Cells were imaged in cell culture medium without additives. Single images were taken with 3-min exposure time. (Scale bar: 20 μm.)
- Download Movie_S02 (MOV) - HeLa cells expressing co lux treated with gramicidin. Cells were imaged in cell culture medium with gramicidin (25 μg/mL). Single images were taken with 2-min exposure time. (Scale bar: 20 μm.)
- Download Movie_S03 (MOV) - HeLa cells expressing co lux treated with DNP. Cells were imaged in cell culture medium with DNP (25 μg/mL). After 8 h, cells were washed and the medium was replaced by fresh cell culture medium without additives. Single images were taken with 3-min exposure time. (Scale bar: 20 μm.)
- Download Movie_S04 (MOV) - HeLa cells expressing co lux imaged in EBSS without glucose. After 12 h, EBSS was replaced by fresh cell culture medium without additives. Single images were taken with 3-min exposure time. (Scale bar: 20 μm.)
Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system
Carola Gregor, Jasmin K. Pape, Klaus C. Gwosch, Tanja Gilat, Steffen J. Sahl, Stefan W. Hell
Proceedings of the National Academy of Sciences Dec 2019, 201913616; DOI: 10.1073/pnas.1913616116
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