A preclinical large-animal model for the assessment of critical-size 
load-bearing bone defect reconstruction

Sparks, David S.; Saifzadeh, Siamak; Savi, Flavia Medeiros; Dlaska, Constantin E.; Berner, Arne; Henkel, Jan; Reichert, Johannes C.; Wullschleger, Martin; Ren, Jiongyu; Cipitria, Amaia; McGovern, Jacqui A.; Steck, Roland; Wagels, Michael; Woodruff, Maria Ann; Schuetz, Michael A.; Hutmacher, Dietmar W.

doi:10.1038/s41596-019-0271-2

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A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction

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Cipitria, Amaia
Amaia Cipitria, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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引用

Sparks, D. S., Saifzadeh, S., Savi, F. M., Dlaska, C. E., Berner, A., Henkel, J., Reichert, J. C., Wullschleger, M., Ren, J., Cipitria, A., McGovern, J. A., Steck, R., Wagels, M., Woodruff, M. A., Schuetz, M. A., & Hutmacher, D. W. (2020). A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction. Nature Protocols, 15(3), 877-924. doi:10.1038/s41596-019-0271-2.

引用: https://hdl.handle.net/21.11116/0000-0005-AADF-1

要旨

Critical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.