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Journal Article

A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2

MPS-Authors
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Maricic,  Tomislav       
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Ayinuer-Petri,  Aximu
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Essel,  Elena       
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Gansauge,  Marie-Theres       
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Kanis,  Philipp       
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Macak,  Dominik
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Riesenberg,  Stephan       
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;
The Leipzig School of Human Origins (IMPRS), Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Bokelmann,  Lukas       
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Zeberg,  Hugo       
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Meyer,  Matthias
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Pääbo,  Svante       
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;
Neandertals and more, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Maricic_A-direct_PLoSOne_2020.pdf
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Citation

Maricic, T., Nickel, O., Ayinuer-Petri, A., Essel, E., Gansauge, M.-T., Kanis, P., et al. (2020). A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2. PLoS One, 15(12): e0244824. doi:10.1371/journal.pone.0244824.


Cite as: https://hdl.handle.net/21.11116/0000-0006-9D73-8
Abstract
SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised
individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast approach to detect SARSCoV-2 in single or pooled gargle lavages (“mouthwashes”). With this approach, we test all staff
at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches could be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.