Low-input Capture-C: A chromosome conformation capture assay to analyze 
chromatin architecture in small numbers of cells

Oudelaar, A. M.; Downes, D. J.; Davies, J. O. J.; Hughes, J. R.

doi:10.21769/BioProtoc.2645

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Low-input Capture-C: A chromosome conformation capture assay to analyze chromatin architecture in small numbers of cells

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Oudelaar, A. M.
Lise Meitner Group Genome Organization and Regulation, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Oudelaar, A. M., Downes, D. J., Davies, J. O. J., & Hughes, J. R. (2017). Low-input Capture-C: A chromosome conformation capture assay to analyze chromatin architecture in small numbers of cells. Bio-protocol, 7(23): e2645. doi:10.21769/BioProtoc.2645.

Cite as: https://hdl.handle.net/21.11116/0000-0007-60F3-A

Abstract

Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.