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Zeitschriftenartikel

A high sensitivity ZENK monoclonal antibody to map neuronal activity in Aves

MPG-Autoren

Malkemper,  Erich Pascal
Max Planck Research Group Neurobiology of Magnetoreception, Center of Advanced European Studies and Research (caesar), Max Planck Society;

Externe Ressourcen

https://www.nature.com/articles/s41598-020-57757-6
(Verlagsversion)

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s41598-020-57757-6.pdf
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Zitation

Nordmann, G. C., Malkemper, E. P., Landler, L., Ushakova, L., Nimpf, S., Heinen, R., et al. (2020). A high sensitivity ZENK monoclonal antibody to map neuronal activity in Aves. Scientific Reports, 10(1): 915. doi:10.1038/s41598-020-57757-6.


Zitierlink: https://hdl.handle.net/21.11116/0000-0007-B4FF-F
Zusammenfassung
The transcription factor ZENK is an immediate early gene that has been employed as a surrogate marker to map neuronal activity in the brain. It has been used in a wide variety of species, however, commercially available antibodies have limited immunoreactivity in birds. To address this issue we generated a new mouse monoclonal antibody, 7B7-A3, raised against ZENK from the rock pigeon (Columba livia). We show that 7B7-A3 labels clZENK in both immunoblots and histological stainings with high sensitivity and selectivity for its target. Using a sound stimulation paradigm we demonstrate that 7B7-A3 can detect activity-dependent ZENK expression at key stations of the central auditory pathway of the pigeon. Finally, we compare staining efficiency across three avian species and confirm that 7B7-A3 is compatible with immunohistochemical detection of ZENK in the rock pigeon, zebra finch, and domestic chicken. Taken together, 7B7-A3 represents a useful tool for the avian neuroscience community to map functional activity in the brain.