date: 2021-01-12T07:01:42Z
pdf:unmappedUnicodeCharsPerPage: 17
pdf:PDFVersion: 1.7
pdf:docinfo:title: Unraveling the In Vivo Protein Corona
xmp:CreatorTool: LaTeX with hyperref package
Keywords: protein corona; nanoparticle; in vivo; serum; plasma; biodistribution
access_permission:modify_annotations: true
access_permission:can_print_degraded: true
subject: Understanding the behavior of nanoparticles upon contact with a physiological environment is of urgent need in order to improve their properties for a successful therapeutic application. Most commonly, the interaction of nanoparticles with plasma proteins are studied under in vitro conditions. However, this has been shown to not reflect the complex situation after in vivo administration. Therefore, here we focused on the investigation of magnetic nanoparticles with blood proteins under in vivo conditions. Importantly, we observed a radically different proteome in vivo in comparison to the in vitro situation underlining the significance of in vivo protein corona studies. Next to this, we found that the in vivo corona profile does not significantly change over time. To mimic the in vivo situation, we established an approach, which we termed ?ex vivo? as it uses whole blood freshly prepared from an animal. Overall, we present a comprehensive analysis focusing on the interaction between nanoparticles and blood proteins under in vivo conditions and how to mimic this situation with our ex vivo approach. This knowledge is needed to characterize the true biological identity of nanoparticles.
dc:creator: Johanna Simon, Gabor Kuhn, Michael Fichter, Stephan Gehring, Katharina Landfester and Volker Mailänder
dcterms:created: 2021-01-12T06:43:48Z
Last-Modified: 2021-01-12T07:01:42Z
dcterms:modified: 2021-01-12T07:01:42Z
dc:format: application/pdf; version=1.7
title: Unraveling the In Vivo Protein Corona
Last-Save-Date: 2021-01-12T07:01:42Z
pdf:docinfo:creator_tool: LaTeX with hyperref package
access_permission:fill_in_form: true
pdf:docinfo:keywords: protein corona; nanoparticle; in vivo; serum; plasma; biodistribution
pdf:docinfo:modified: 2021-01-12T07:01:42Z
meta:save-date: 2021-01-12T07:01:42Z
pdf:encrypted: false
dc:title: Unraveling the In Vivo Protein Corona
modified: 2021-01-12T07:01:42Z
cp:subject: Understanding the behavior of nanoparticles upon contact with a physiological environment is of urgent need in order to improve their properties for a successful therapeutic application. Most commonly, the interaction of nanoparticles with plasma proteins are studied under in vitro conditions. However, this has been shown to not reflect the complex situation after in vivo administration. Therefore, here we focused on the investigation of magnetic nanoparticles with blood proteins under in vivo conditions. Importantly, we observed a radically different proteome in vivo in comparison to the in vitro situation underlining the significance of in vivo protein corona studies. Next to this, we found that the in vivo corona profile does not significantly change over time. To mimic the in vivo situation, we established an approach, which we termed ?ex vivo? as it uses whole blood freshly prepared from an animal. Overall, we present a comprehensive analysis focusing on the interaction between nanoparticles and blood proteins under in vivo conditions and how to mimic this situation with our ex vivo approach. This knowledge is needed to characterize the true biological identity of nanoparticles.
pdf:docinfo:subject: Understanding the behavior of nanoparticles upon contact with a physiological environment is of urgent need in order to improve their properties for a successful therapeutic application. Most commonly, the interaction of nanoparticles with plasma proteins are studied under in vitro conditions. However, this has been shown to not reflect the complex situation after in vivo administration. Therefore, here we focused on the investigation of magnetic nanoparticles with blood proteins under in vivo conditions. Importantly, we observed a radically different proteome in vivo in comparison to the in vitro situation underlining the significance of in vivo protein corona studies. Next to this, we found that the in vivo corona profile does not significantly change over time. To mimic the in vivo situation, we established an approach, which we termed ?ex vivo? as it uses whole blood freshly prepared from an animal. Overall, we present a comprehensive analysis focusing on the interaction between nanoparticles and blood proteins under in vivo conditions and how to mimic this situation with our ex vivo approach. This knowledge is needed to characterize the true biological identity of nanoparticles.
Content-Type: application/pdf
pdf:docinfo:creator: Johanna Simon, Gabor Kuhn, Michael Fichter, Stephan Gehring, Katharina Landfester and Volker Mailänder
X-Parsed-By: org.apache.tika.parser.DefaultParser
creator: Johanna Simon, Gabor Kuhn, Michael Fichter, Stephan Gehring, Katharina Landfester and Volker Mailänder
meta:author: Johanna Simon, Gabor Kuhn, Michael Fichter, Stephan Gehring, Katharina Landfester and Volker Mailänder
dc:subject: protein corona; nanoparticle; in vivo; serum; plasma; biodistribution
meta:creation-date: 2021-01-12T06:43:48Z
created: 2021-01-12T06:43:48Z
access_permission:extract_for_accessibility: true
access_permission:assemble_document: true
xmpTPg:NPages: 12
Creation-Date: 2021-01-12T06:43:48Z
pdf:charsPerPage: 3860
access_permission:extract_content: true
access_permission:can_print: true
meta:keyword: protein corona; nanoparticle; in vivo; serum; plasma; biodistribution
Author: Johanna Simon, Gabor Kuhn, Michael Fichter, Stephan Gehring, Katharina Landfester and Volker Mailänder
producer: pdfTeX-1.40.18
access_permission:can_modify: true
pdf:docinfo:producer: pdfTeX-1.40.18
pdf:docinfo:created: 2021-01-12T06:43:48Z